Detailed information of TA system TA095597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 127108..127702
• Replicon: plasmid p266917_2_01
• Accession: NZ_CP026724
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C4R22_RS29535
• Protein ID: WP_001403920.1
• Coordinates: 127427..127702 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: C4R22_RS29530
• Protein ID: WP_001261274.1
• Coordinates: 127108..127338 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS29515	122653..123411	-	759	WP_023063772.1	hypothetical protein	-
C4R22_RS29520	123423..123914	-	492	WP_000528930.1	hypothetical protein	-
C4R22_RS29525	124288..126909	+	2622	WP_023063774.1	ABC transporter	-
C4R22_RS29530	127108..127338	+	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C4R22_RS29535	127427..127702	+	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
C4R22_RS31700	127707..127924	-	218	Protein_156	transposase	-
C4R22_RS29540	127925..128455	-	531	WP_023063775.1	GNAT family N-acetyltransferase	-
C4R22_RS29545	128459..128728	-	270	WP_001275013.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / aap/aspU / aggA / aggB / aggC / aggD	1..129627	129627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T95597 WP_001403920.1 NZ_CP026724:127427-127702 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T95597 NZ_CP026724:127427-127702 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT95597 WP_001261274.1 NZ_CP026724:127108-127338 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT95597 NZ_CP026724:127108-127338 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1