Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 118047..118572 Replicon plasmid p266917_2_01
Accession NZ_CP026724
Organism Escherichia coli strain 266917_2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C4R22_RS29485 Protein ID WP_001159871.1
Coordinates 118047..118352 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C4R22_RS29490 Protein ID WP_000813639.1
Coordinates 118354..118572 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4R22_RS29460 114220..114636 - 417 WP_001278815.1 plasmid partitioning/stability family protein -
C4R22_RS29465 114629..115609 - 981 WP_001403375.1 plasmid segregation protein ParM -
C4R22_RS29470 116022..116330 - 309 WP_000030204.1 molecular chaperone GroEL -
C4R22_RS29475 116417..117061 - 645 WP_001144036.1 ParA family protein -
C4R22_RS29480 117240..118046 - 807 WP_024210383.1 site-specific integrase -
C4R22_RS29485 118047..118352 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C4R22_RS29490 118354..118572 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C4R22_RS29500 119390..119680 - 291 WP_000648897.1 hypothetical protein -
C4R22_RS29505 119680..120804 - 1125 WP_000386705.1 DUF4238 domain-containing protein -
C4R22_RS29510 120839..122431 - 1593 WP_001350003.1 hypothetical protein -
C4R22_RS29515 122653..123411 - 759 WP_023063772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / aap/aspU / aggA / aggB / aggC / aggD 1..129627 129627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T95596 WP_001159871.1 NZ_CP026724:c118352-118047 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T95596 NZ_CP026724:c118352-118047 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT95596 WP_000813639.1 NZ_CP026724:c118572-118354 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT95596 NZ_CP026724:c118572-118354 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References