Detailed information of TA system TA095587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 41926..42473
• Replicon: plasmid p266917_2_01
• Accession: NZ_CP026724
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C4R22_RS28935
• Protein ID: WP_001402901.1
• Coordinates: 41926..42207 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: C4R22_RS28940
• Protein ID: WP_000079941.1
• Coordinates: 42204..42473 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS28910	37858..38571	+	714	WP_032269703.1	MgtC/SapB family protein	-
C4R22_RS28915	38728..38967	-	240	Protein_43	IS4 family transposase	-
C4R22_RS28920	39260..40282	-	1023	WP_089643065.1	IS110 family transposase	-
C4R22_RS28925	40442..40924	-	483	WP_001402899.1	hypothetical protein	-
C4R22_RS28930	41041..41880	-	840	WP_001402900.1	3'-5' exonuclease	-
C4R22_RS28935	41926..42207	-	282	WP_001402901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C4R22_RS28940	42204..42473	-	270	WP_000079941.1	hypothetical protein	Antitoxin
C4R22_RS28955	43390..44247	-	858	WP_000130641.1	incFII family plasmid replication initiator RepA	-
C4R22_RS28960	44240..44314	-	75	WP_031943482.1	RepA leader peptide Tap	-
C4R22_RS31930	44311..44445	-	135	Protein_51	protein CopA/IncA	-
C4R22_RS28970	44552..44806	-	255	WP_089643063.1	replication regulatory protein RepA	-
C4R22_RS28975	45103..45237	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	-
-	45283..45334	+	52	NuclAT_1	-	-
-	45283..45334	+	52	NuclAT_1	-	-
-	45283..45334	+	52	NuclAT_1	-	-
-	45283..45334	+	52	NuclAT_1	-	-
C4R22_RS28980	45301..45587	-	287	Protein_54	DUF2726 domain-containing protein	-
C4R22_RS28990	46099..46311	-	213	WP_104858475.1	ANR family transcriptional regulator	-
C4R22_RS28995	46443..47003	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / aap/aspU / aggA / aggB / aggC / aggD	1..129627	129627
	-	flank	IS/Tn	-	-	39260..40282	1022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10875.53 Da
• Isoelectric Point: 8.4615

>T95587 WP_001402901.1 NZ_CP026724:c42207-41926 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSQWGKV
YILRVLHTTQKWP

Nucleotide

• Length: 282 bp

>T95587 NZ_CP026724:c42207-41926 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTACACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT95587 WP_000079941.1 NZ_CP026724:c42473-42204 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT95587 NZ_CP026724:c42473-42204 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5