Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5251..5810 Replicon plasmid p266917_2_01
Accession NZ_CP026724
Organism Escherichia coli strain 266917_2

Toxin (Protein)


Gene name relE Uniprot ID G4VUF8
Locus tag C4R22_RS28720 Protein ID WP_000421273.1
Coordinates 5251..5526 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag C4R22_RS28725 Protein ID WP_001387467.1
Coordinates 5526..5810 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4R22_RS28680 1301..2041 - 741 WP_001066940.1 tyrosine-type recombinase/integrase -
C4R22_RS28695 2689..2994 - 306 WP_089643027.1 type II toxin-antitoxin system ParD family antitoxin -
C4R22_RS28705 3487..4656 + 1170 WP_001403369.1 hypothetical protein -
C4R22_RS28710 4689..4817 + 129 Protein_4 IS481 family transposase -
C4R22_RS28715 4852..5145 - 294 WP_001105065.1 hypothetical protein -
C4R22_RS28720 5251..5526 - 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C4R22_RS28725 5526..5810 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
C4R22_RS28730 6415..7167 + 753 WP_001403368.1 IclR family transcriptional regulator -
C4R22_RS28735 7213..8178 - 966 WP_023063778.1 2-keto-3-deoxygluconate transporter -
C4R22_RS28740 8211..8591 - 381 WP_001403366.1 Rid family detoxifying hydrolase -
C4R22_RS28745 8616..9506 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
C4R22_RS28750 9859..10425 + 567 Protein_12 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pic / aap/aspU / aggA / aggB / aggC / aggD 1..129627 129627


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10765.45 Da        Isoelectric Point: 9.9324

>T95586 WP_000421273.1 NZ_CP026724:c5526-5251 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T95586 NZ_CP026724:c5526-5251 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT95586 WP_001387467.1 NZ_CP026724:c5810-5526 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT95586 NZ_CP026724:c5810-5526 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6H1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References