Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4301848..4302466 Replicon chromosome
Accession NZ_CP026723
Organism Escherichia coli strain 266917_2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C4R22_RS22085 Protein ID WP_001291435.1
Coordinates 4301848..4302066 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C4R22_RS22090 Protein ID WP_000344800.1
Coordinates 4302092..4302466 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4R22_RS22050 4297137..4297709 + 573 WP_000779831.1 YbaY family lipoprotein -
C4R22_RS22055 4297740..4298051 - 312 WP_000409911.1 MGMT family protein -
C4R22_RS22065 4298430..4298783 + 354 WP_001550741.1 DUF1428 family protein -
C4R22_RS22070 4298825..4300375 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C4R22_RS22075 4300539..4301009 - 471 WP_000136192.1 YlaC family protein -
C4R22_RS22080 4301125..4301676 - 552 WP_000102564.1 maltose O-acetyltransferase -
C4R22_RS22085 4301848..4302066 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C4R22_RS22090 4302092..4302466 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C4R22_RS22095 4303012..4306161 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
C4R22_RS22100 4306184..4307377 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95581 WP_001291435.1 NZ_CP026723:c4302066-4301848 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T95581 NZ_CP026723:c4302066-4301848 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT95581 WP_000344800.1 NZ_CP026723:c4302466-4302092 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT95581 NZ_CP026723:c4302466-4302092 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References