Detailed information of TA system TA095578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3837779..3838203
• Replicon: chromosome
• Accession: NZ_CP026723
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C4R22_RS31815
• Protein ID: WP_001586653.1
• Coordinates: 3838015..3838203 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: C4R22_RS19760
• Protein ID: WP_000125566.1
• Coordinates: 3837779..3838012 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS19735	3832852..3833139	+	288	WP_001596509.1	ferredoxin-like protein FixX	-
C4R22_RS19740	3833198..3834529	+	1332	WP_001183198.1	MFS transporter	-
C4R22_RS19745	3834637..3835167	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
C4R22_RS19750	3835160..3837022	+	1863	WP_000377117.1	glutathione-regulated potassium-efflux system protein KefC	-
C4R22_RS19755	3837214..3837693	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
C4R22_RS19760	3837779..3838012	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C4R22_RS31815	3838015..3838203	+	189	WP_001586653.1	CcdB family protein	Toxin
C4R22_RS31820	3838200..3838316	+	117	WP_001389634.1	CcdB family protein	-
C4R22_RS19770	3838313..3839161	-	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
C4R22_RS19775	3839168..3839545	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
C4R22_RS19780	3839548..3840369	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
C4R22_RS19785	3840366..3841355	-	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
C4R22_RS19790	3841355..3842641	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7029.22 Da
• Isoelectric Point: 8.7938

>T95578 WP_001586653.1 NZ_CP026723:3838015-3838203 [Escherichia coli]
MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T95578 NZ_CP026723:3838015-3838203 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGTAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCAGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT95578 WP_000125566.1 NZ_CP026723:3837779-3838012 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT95578 NZ_CP026723:3837779-3838012 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9