Detailed information of TA system TA095565

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 926089..926921
• Replicon: chromosome
• Accession: NZ_CP026723
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: C4R22_RS04835
• Protein ID: WP_000854765.1
• Coordinates: 926547..926921 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: C4R22_RS04830
• Protein ID: WP_001295723.1
• Coordinates: 926089..926457 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS04805	923517..924335	+	819	WP_104858308.1	DUF945 domain-containing protein	-
C4R22_RS04815	924677..925150	+	474	WP_000855059.1	antirestriction protein	-
C4R22_RS04820	925166..925642	+	477	WP_001186726.1	RadC family protein	-
C4R22_RS04825	925705..925926	+	222	WP_000692345.1	DUF987 domain-containing protein	-
C4R22_RS04830	926089..926457	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C4R22_RS04835	926547..926921	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
C4R22_RS04840	926918..927124	+	207	WP_000976829.1	hypothetical protein	-
C4R22_RS31755	927137..927250	+	114	WP_001161660.1	DUF957 domain-containing protein	-
C4R22_RS31760	928115..928504	+	390	WP_077249349.1	transposase	-
C4R22_RS04855	929241..929570	-	330	WP_000450409.1	DUF496 family protein	-
C4R22_RS04860	929742..930800	-	1059	WP_001200891.1	FUSC family protein	-
C4R22_RS04865	930999..931472	-	474	WP_001105412.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T95565 WP_000854765.1 NZ_CP026723:926547-926921 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T95565 NZ_CP026723:926547-926921 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTGACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT95565 WP_001295723.1 NZ_CP026723:926089-926457 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT95565 NZ_CP026723:926089-926457 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52