Detailed information of TA system TA095551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 44637..45162
• Replicon: plasmid unitig_2_pilon
• Accession: NZ_CP026720
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: AM439_RS25440
• Protein ID: WP_016946795.1
• Coordinates: 44637..44942 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: AM439_RS25445
• Protein ID: WP_004197642.1
• Coordinates: 44944..45162 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS25410 (AM439_25410)	40442..40711	+	270	WP_000339857.1	hypothetical protein	-
AM439_RS25420 (AM439_25420)	41068..41934	-	867	WP_004118283.1	replication initiation protein	-
AM439_RS25945	42469..42573	+	105	WP_032409716.1	hypothetical protein	-
AM439_RS25425 (AM439_25425)	42702..42959	+	258	WP_000764642.1	hypothetical protein	-
AM439_RS25430 (AM439_25430)	43017..43793	-	777	WP_049245386.1	site-specific integrase	-
AM439_RS25435 (AM439_25435)	43796..44479	-	684	WP_023280892.1	hypothetical protein	-
AM439_RS25440 (AM439_25440)	44637..44942	-	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
AM439_RS25445 (AM439_25445)	44944..45162	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AM439_RS25460 (AM439_25460)	45757..46005	+	249	WP_032415726.1	hypothetical protein	-
AM439_RS25465 (AM439_25465)	46002..47234	+	1233	WP_004200905.1	hypothetical protein	-
AM439_RS25470 (AM439_25470)	47368..47859	+	492	WP_004200903.1	hypothetical protein	-
AM439_RS26300	48862..48984	-	123	WP_254913144.1	hypothetical protein	-
AM439_RS25485 (AM439_25485)	49084..49434	-	351	WP_004200891.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkC / mrkB / mrkA / mrkJ / mrkF / mrkD	1..77822	77822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T95551 WP_016946795.1 NZ_CP026720:c44942-44637 [Enterobacter cloacae complex sp.]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T95551 NZ_CP026720:c44942-44637 [Enterobacter cloacae complex sp.]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT95551 WP_004197642.1 NZ_CP026720:c45162-44944 [Enterobacter cloacae complex sp.]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT95551 NZ_CP026720:c45162-44944 [Enterobacter cloacae complex sp.]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA