Detailed information of TA system TA095550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4700001..4700740
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A3L9PBP9
• Locus tag: AM439_RS23315
• Protein ID: WP_003857133.1
• Coordinates: 4700001..4700486 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: AM439_RS23320
• Protein ID: WP_049132942.1
• Coordinates: 4700474..4700740 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS23290 (AM439_23290)	4695508..4695933	-	426	WP_000065802.1	glutaredoxin-dependent arsenate reductase	-
AM439_RS23295 (AM439_23295)	4695946..4697235	-	1290	WP_000922628.1	arsenic transporter	-
AM439_RS23300 (AM439_23300)	4697281..4697601	-	321	WP_000941305.1	transcriptional regulator	-
AM439_RS23305 (AM439_23305)	4697688..4698392	+	705	WP_000130816.1	arsenical resistance protein ArsH	-
AM439_RS23310 (AM439_23310)	4698425..4699828	-	1404	WP_000125668.1	ISNCY family transposase	-
AM439_RS23315 (AM439_23315)	4700001..4700486	-	486	WP_003857133.1	GNAT family N-acetyltransferase	Toxin
AM439_RS23320 (AM439_23320)	4700474..4700740	-	267	WP_049132942.1	DUF1778 domain-containing protein	Antitoxin
AM439_RS23325 (AM439_23325)	4700804..4701722	-	919	Protein_4452	LysR family transcriptional regulator	-
AM439_RS23330 (AM439_23330)	4701852..4703240	+	1389	WP_049132943.1	MFS transporter	-
AM439_RS23335 (AM439_23335)	4703262..4704257	-	996	WP_049132944.1	DUF2891 domain-containing protein	-
AM439_RS23340 (AM439_23340)	4704267..4705253	-	987	WP_017382341.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4698425..4699828	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17540.23 Da
• Isoelectric Point: 9.9658

>T95550 WP_003857133.1 NZ_CP026719:c4700486-4700001 [Enterobacter cloacae complex sp.]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T95550 NZ_CP026719:c4700486-4700001 [Enterobacter cloacae complex sp.]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGTGTAGCAGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10104.52 Da
• Isoelectric Point: 4.3837

>AT95550 WP_049132942.1 NZ_CP026719:c4700740-4700474 [Enterobacter cloacae complex sp.]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRIFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT95550 NZ_CP026719:c4700740-4700474 [Enterobacter cloacae complex sp.]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTATATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A3L9PBP9