Detailed information of TA system TA095549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4099832..4100443
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: AM439_RS20325
• Protein ID: WP_012906750.1
• Coordinates: 4100264..4100443 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM439_RS20320
• Protein ID: WP_023303479.1
• Coordinates: 4099832..4100242 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS20300 (AM439_20300)	4095666..4096217	+	552	WP_023303475.1	phage tail protein I	-
AM439_RS26240	4096220..4098337	+	2118	WP_255411898.1	phage tail protein	-
AM439_RS20310 (AM439_20310)	4098340..4098855	+	516	WP_023303477.1	tail fiber assembly protein	-
AM439_RS20315 (AM439_20315)	4098852..4099718	-	867	WP_023303478.1	beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase	-
AM439_RS20320 (AM439_20320)	4099832..4100242	-	411	WP_023303479.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM439_RS20325 (AM439_20325)	4100264..4100443	-	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM439_RS20330 (AM439_20330)	4100669..4101532	-	864	WP_045340850.1	spermidine/putrescine ABC transporter permease PotB	-
AM439_RS20335 (AM439_20335)	4101516..4102664	-	1149	WP_162837914.1	spermidine/putrescine ABC transporter ATP-binding protein PotA	-
AM439_RS20340 (AM439_20340)	4102902..4104125	+	1224	WP_049132768.1	peptidase T	-
AM439_RS20345 (AM439_20345)	4104223..4104714	+	492	WP_049132766.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T95549 WP_012906750.1 NZ_CP026719:c4100443-4100264 [Enterobacter cloacae complex sp.]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T95549 NZ_CP026719:c4100443-4100264 [Enterobacter cloacae complex sp.]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14858.83 Da
• Isoelectric Point: 4.2855

>AT95549 WP_023303479.1 NZ_CP026719:c4100242-4099832 [Enterobacter cloacae complex sp.]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDDYQDAIESVREAIEAHIELLVEDGEAVPEATTIENWLSDPEYAGVVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVVGLQKR

Nucleotide

• Length: 411 bp

>AT95549 NZ_CP026719:c4100242-4099832 [Enterobacter cloacae complex sp.]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATTGAAAGCGTACGTGAAGCGATCGAAGCACATATTGAGTTGCTGGTTGAAGATGGCG
AGGCCGTGCCCGAAGCAACGACCATCGAAAACTGGCTTTCCGATCCTGAATATGCCGGTGTGGTATGGGCCCTGGTTGAT
GTGGATATAACGCGCCTGATGGGGAAAGCGGAAAAAATCAATGTTACACTTCCGTCTCTGTTGATCCGTCGTATCGATCA
GTTCGTGGCCGCGCATCCAGAATACGGCAGTCGGTCTGGCTTCCTGTCCCGCGTCGCAGCAGATAAAGTTGTCGGACTAC
AAAAACGATAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4