Detailed information of TA system TA095548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4059283..4059894
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: AM439_RS20050
• Protein ID: WP_012906750.1
• Coordinates: 4059715..4059894 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AM439_RS20045
• Protein ID: WP_023303479.1
• Coordinates: 4059283..4059693 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS20025 (AM439_20025)	4055117..4055668	+	552	WP_023303475.1	phage tail protein I	-
AM439_RS26205	4055671..4057788	+	2118	WP_255411898.1	phage tail protein	-
AM439_RS20035 (AM439_20035)	4057791..4058306	+	516	WP_023303477.1	tail fiber assembly protein	-
AM439_RS20040 (AM439_20040)	4058303..4059169	-	867	WP_023303478.1	beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase	-
AM439_RS20045 (AM439_20045)	4059283..4059693	-	411	WP_023303479.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM439_RS20050 (AM439_20050)	4059715..4059894	-	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM439_RS20055 (AM439_20055)	4060318..4061436	-	1119	WP_104879842.1	phage integrase Arm DNA-binding domain-containing protein	-
AM439_RS20060 (AM439_20060)	4061411..4061674	-	264	WP_023303435.1	hypothetical protein	-
AM439_RS26210 (AM439_20065)	4061742..4064306	-	2565	WP_104879843.1	exonuclease	-
AM439_RS20075 (AM439_20075)	4064447..4064743	-	297	WP_104879844.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4058303..4077280	18977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T95548 WP_012906750.1 NZ_CP026719:c4059894-4059715 [Enterobacter cloacae complex sp.]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T95548 NZ_CP026719:c4059894-4059715 [Enterobacter cloacae complex sp.]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14858.83 Da
• Isoelectric Point: 4.2855

>AT95548 WP_023303479.1 NZ_CP026719:c4059693-4059283 [Enterobacter cloacae complex sp.]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDDYQDAIESVREAIEAHIELLVEDGEAVPEATTIENWLSDPEYAGVVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVVGLQKR

Nucleotide

• Length: 411 bp

>AT95548 NZ_CP026719:c4059693-4059283 [Enterobacter cloacae complex sp.]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATTGAAAGCGTACGTGAAGCGATCGAAGCACATATTGAGTTGCTGGTTGAAGATGGCG
AGGCCGTGCCCGAAGCAACGACCATCGAAAACTGGCTTTCCGATCCTGAATATGCCGGTGTGGTATGGGCCCTGGTTGAT
GTGGATATAACGCGCCTGATGGGGAAAGCGGAAAAAATCAATGTTACACTTCCGTCTCTGTTGATCCGTCGTATCGATCA
GTTCGTGGCCGCGCATCCAGAATACGGCAGTCGGTCTGGCTTCCTGTCCCGCGTCGCAGCAGATAAAGTTGTCGGACTAC
AAAAACGATAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4