Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3278551..3279171 Replicon chromosome
Accession NZ_CP026719
Organism Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag AM439_RS16095 Protein ID WP_015571250.1
Coordinates 3278551..3278769 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag AM439_RS16100 Protein ID WP_006809850.1
Coordinates 3278797..3279171 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM439_RS16065 (AM439_16065) 3274563..3274823 + 261 WP_015571255.1 type B 50S ribosomal protein L31 -
AM439_RS16070 (AM439_16070) 3274826..3274966 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM439_RS16075 (AM439_16075) 3274963..3275673 - 711 WP_017383207.1 GNAT family protein -
AM439_RS16080 (AM439_16080) 3275775..3277235 + 1461 WP_049134104.1 PLP-dependent aminotransferase family protein -
AM439_RS16085 (AM439_16085) 3277207..3277674 - 468 WP_015571252.1 YlaC family protein -
AM439_RS16090 (AM439_16090) 3277791..3278342 - 552 WP_015571251.1 maltose O-acetyltransferase -
AM439_RS16095 (AM439_16095) 3278551..3278769 - 219 WP_015571250.1 HHA domain-containing protein Toxin
AM439_RS16100 (AM439_16100) 3278797..3279171 - 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
AM439_RS16105 (AM439_16105) 3279682..3282828 - 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
AM439_RS16110 (AM439_16110) 3282851..3284044 - 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T95546 WP_015571250.1 NZ_CP026719:c3278769-3278551 [Enterobacter cloacae complex sp.]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T95546 NZ_CP026719:c3278769-3278551 [Enterobacter cloacae complex sp.]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTTTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT95546 WP_006809850.1 NZ_CP026719:c3279171-3278797 [Enterobacter cloacae complex sp.]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT95546 NZ_CP026719:c3279171-3278797 [Enterobacter cloacae complex sp.]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACTTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References