Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2800091..2800667 Replicon chromosome
Accession NZ_CP026719
Organism Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)


Gene name shpA Uniprot ID A0A800YKM1
Locus tag AM439_RS13815 Protein ID WP_015572580.1
Coordinates 2800091..2800378 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A801DSF4
Locus tag AM439_RS13820 Protein ID WP_017694570.1
Coordinates 2800365..2800667 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM439_RS13790 (AM439_13790) 2795184..2795903 + 720 WP_049132589.1 winged helix-turn-helix domain-containing protein -
AM439_RS13795 (AM439_13795) 2796053..2796523 + 471 WP_049133317.1 MarR family transcriptional regulator -
AM439_RS13800 (AM439_13800) 2796520..2797587 + 1068 WP_104879798.1 HlyD family secretion protein -
AM439_RS13805 (AM439_13805) 2797577..2798653 + 1077 WP_015572578.1 DUF2955 domain-containing protein -
AM439_RS13810 (AM439_13810) 2798650..2799921 - 1272 WP_049133321.1 DUF445 domain-containing protein -
AM439_RS13815 (AM439_13815) 2800091..2800378 + 288 WP_015572580.1 BrnT family toxin Toxin
AM439_RS13820 (AM439_13820) 2800365..2800667 + 303 WP_017694570.1 BrnA antitoxin family protein Antitoxin
AM439_RS13825 (AM439_13825) 2800696..2801334 - 639 WP_047736941.1 LysE family translocator -
AM439_RS13830 (AM439_13830) 2801373..2802125 - 753 WP_015572582.1 AraC family transcriptional regulator -
AM439_RS13835 (AM439_13835) 2802279..2803649 + 1371 WP_049133324.1 NAD-dependent succinate-semialdehyde dehydrogenase -
AM439_RS13840 (AM439_13840) 2803827..2804372 - 546 WP_006810254.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11296.81 Da        Isoelectric Point: 7.3587

>T95545 WP_015572580.1 NZ_CP026719:2800091-2800378 [Enterobacter cloacae complex sp.]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Download         Length: 288 bp

>T95545 NZ_CP026719:2800091-2800378 [Enterobacter cloacae complex sp.]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11482.13 Da        Isoelectric Point: 10.4094

>AT95545 WP_017694570.1 NZ_CP026719:2800365-2800667 [Enterobacter cloacae complex sp.]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT95545 NZ_CP026719:2800365-2800667 [Enterobacter cloacae complex sp.]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References