Detailed information of TA system TA095543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2035886..2036502
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AM439_RS10085
• Protein ID: WP_077266491.1
• Coordinates: 2035886..2036257 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: AM439_RS10090
• Protein ID: WP_015569912.1
• Coordinates: 2036260..2036502 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS10070 (AM439_10070)	2033386..2034288	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
AM439_RS10075 (AM439_10075)	2034285..2034920	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
AM439_RS10080 (AM439_10080)	2034917..2035846	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
AM439_RS10085 (AM439_10085)	2035886..2036257	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM439_RS10090 (AM439_10090)	2036260..2036502	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM439_RS10095 (AM439_10095)	2036701..2037621	+	921	WP_017382675.1	alpha/beta hydrolase	-
AM439_RS10100 (AM439_10100)	2037630..2038571	-	942	WP_049134693.1	fatty acid biosynthesis protein FabY	-
AM439_RS10105 (AM439_10105)	2038616..2039053	-	438	WP_049134694.1	D-aminoacyl-tRNA deacylase	-
AM439_RS10110 (AM439_10110)	2039050..2039931	-	882	WP_003861949.1	virulence factor BrkB family protein	-
AM439_RS10115 (AM439_10115)	2039925..2040524	-	600	WP_017694048.1	glucose-1-phosphatase	-
AM439_RS10120 (AM439_10120)	2040643..2041443	-	801	WP_049134695.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T95543 WP_077266491.1 NZ_CP026719:c2036257-2035886 [Enterobacter cloacae complex sp.]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T95543 NZ_CP026719:c2036257-2035886 [Enterobacter cloacae complex sp.]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT95543 WP_015569912.1 NZ_CP026719:c2036502-2036260 [Enterobacter cloacae complex sp.]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT95543 NZ_CP026719:c2036502-2036260 [Enterobacter cloacae complex sp.]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3