Detailed information of TA system TA095542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1912211..1912815
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AM439_RS09505
• Protein ID: WP_072047912.1
• Coordinates: 1912630..1912815 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2G4ZRB5
• Locus tag: AM439_RS09500
• Protein ID: WP_023295606.1
• Coordinates: 1912211..1912615 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS09470 (AM439_09470)	1907782..1909287	+	1506	WP_049134657.1	FGGY-family carbohydrate kinase	-
AM439_RS09475 (AM439_09475)	1909284..1909937	+	654	WP_045334687.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
AM439_RS09480 (AM439_09480)	1909940..1910800	+	861	WP_017693794.1	L-ribulose-5-phosphate 3-epimerase	-
AM439_RS09485 (AM439_09485)	1910794..1911489	+	696	WP_023295602.1	L-ribulose-5-phosphate 4-epimerase	-
AM439_RS09490 (AM439_09490)	1911490..1911777	-	288	Protein_1801	helix-turn-helix domain-containing protein	-
AM439_RS09495 (AM439_09495)	1911772..1912155	+	384	Protein_1802	glycoside hydrolase family 127 protein	-
AM439_RS09500 (AM439_09500)	1912211..1912615	-	405	WP_023295606.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM439_RS09505 (AM439_09505)	1912630..1912815	-	186	WP_072047912.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM439_RS09510 (AM439_09510)	1913065..1914603	-	1539	WP_006808612.1	aldehyde dehydrogenase AldB	-
AM439_RS09515 (AM439_09515)	1914770..1915654	+	885	WP_015572761.1	ROK family protein	-
AM439_RS09520 (AM439_09520)	1915658..1917496	-	1839	WP_049134659.1	selenocysteine-specific translation elongation factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6812.02 Da
• Isoelectric Point: 11.5191

>T95542 WP_072047912.1 NZ_CP026719:c1912815-1912630 [Enterobacter cloacae complex sp.]
VKSADIIAVLVSHGWKCVRTKGSHHQFRHPVQKGLVTVPHPKKDIKSGTLAQIWRQAGIKH

Nucleotide

• Length: 186 bp

>T95542 NZ_CP026719:c1912815-1912630 [Enterobacter cloacae complex sp.]
GTGAAAAGTGCGGATATCATTGCTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGAAGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTAACCGTTCCGCATCCTAAAAAGGACATTAAATCGGGCACTCTCGCACAGATCT
GGCGACAGGCGGGCATTAAACACTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14954.71 Da
• Isoelectric Point: 4.2329

>AT95542 WP_023295606.1 NZ_CP026719:c1912615-1912211 [Enterobacter cloacae complex sp.]
MFYPAYIHSDSDGSASGFFPDVPGCFFAGDSLDDAFQDARDALTAHFEALFEMDEALPLPGSVEVHLENQPDDFTGGQWL
LVDINMKQFDGRAERINITMPRRLLVKIDSFVSEHPQFGNRSAFLAEAARRVLP

Nucleotide

• Length: 405 bp

>AT95542 NZ_CP026719:c1912615-1912211 [Enterobacter cloacae complex sp.]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGCGGTTTCTTCCCCGACGTTCCGGGATGTTTTTT
TGCGGGGGATTCACTTGATGACGCTTTTCAGGATGCACGTGATGCGCTCACGGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGAAGCATTGCCTCTCCCGGGTAGTGTCGAAGTGCACCTTGAAAATCAACCTGACGACTTCACCGGCGGGCAATGGTTG
CTGGTGGATATTAATATGAAGCAATTCGACGGCAGAGCCGAACGCATCAACATCACGATGCCTCGACGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCTCAATTCGGCAATCGAAGCGCATTTCTGGCCGAGGCAGCACGTCGCGTGCTGC
CGTAG

Structures

Antitoxin

• AlphaFold DB: A0A2G4ZRB5