Detailed information of TA system TA095541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1242936..1243714
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: AM439_RS06040
• Protein ID: WP_049133171.1
• Coordinates: 1242936..1243424 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: AM439_RS06045
• Protein ID: WP_049133169.1
• Coordinates: 1243421..1243714 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS06035 (AM439_06030)	1239113..1242856	+	3744	WP_049133172.1	hypothetical protein	-
AM439_RS06040 (AM439_06035)	1242936..1243424	-	489	WP_049133171.1	GNAT family N-acetyltransferase	Toxin
AM439_RS06045 (AM439_06040)	1243421..1243714	-	294	WP_049133169.1	DUF1778 domain-containing protein	Antitoxin
AM439_RS06050 (AM439_06045)	1244040..1245824	-	1785	WP_049133167.1	hybrid sensor histidine kinase/response regulator	-
AM439_RS06055 (AM439_06050)	1245821..1246453	-	633	WP_017692678.1	response regulator transcription factor	-
AM439_RS06060 (AM439_06055)	1246629..1246943	+	315	WP_017382737.1	lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1207770..1246943	39173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17523.35 Da
• Isoelectric Point: 7.7539

>T95541 WP_049133171.1 NZ_CP026719:c1243424-1242936 [Enterobacter cloacae complex sp.]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVTTNTAPGRFRRNMPDP
IPVVVLGRLAVDRSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T95541 NZ_CP026719:c1243424-1242936 [Enterobacter cloacae complex sp.]
ATGATCTCCGCGCCAGAACCGCTTCACGCCGGGCATATTCTTACCCCGTTTTGTTGTGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGTACCTTTGTGTGTTGCGGCAGTGATTCTAACGTAC
TGGCGTATTACTCGCTGGCGTCCAGCGCGGTAACAACAAACACCGCCCCCGGCCGCTTTCGTCGCAATATGCCCGATCCG
ATCCCTGTTGTGGTGTTGGGACGTCTGGCGGTGGATCGGTCGCTACATGGGCAGGGCGTCGGGCGGGCGCTGGTGAGGGA
TGCCGGACTGCGGGTGATTCAGGTGGCGGAAACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGCTGATGGTGACGTTGGGGGATTTGGTA
CATGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10875.52 Da
• Isoelectric Point: 9.8590

>AT95541 WP_049133169.1 NZ_CP026719:c1243714-1243421 [Enterobacter cloacae complex sp.]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQAFLTRLDQAPAPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT95541 NZ_CP026719:c1243714-1243421 [Enterobacter cloacae complex sp.]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGCTGCAAAAGCCAGAGGGAAAAACCGCACTGATTTTGTGCTGGAAGCTGCCCGCGCTGCTGCGGAGGAAGCACTGA
TCGAGCAACGTATCATCATGGCCGATCCGGAGGCTTATCAGGCATTTCTGACTCGGCTGGATCAGGCTCCAGCGCCAAAC
GCCGCGTTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA