Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 445800..447583 Replicon chromosome
Accession NZ_CP026719
Organism Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)


Gene name hipA Uniprot ID A0A2G5A822
Locus tag AM439_RS02240 Protein ID WP_045333653.1
Coordinates 445800..447149 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag AM439_RS02245 Protein ID WP_049134548.1
Coordinates 447146..447583 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM439_RS02215 (AM439_02210) 441623..442558 - 936 WP_015572323.1 tRNA dihydrouridine(16) synthase DusC -
AM439_RS02220 (AM439_02215) 442727..443122 + 396 WP_003859308.1 CidA/LrgA family protein -
AM439_RS02225 (AM439_02220) 443119..443814 + 696 WP_015572322.1 CidB/LrgB family autolysis modulator -
AM439_RS02230 (AM439_02225) 443943..444827 + 885 WP_049134549.1 cytidine deaminase -
AM439_RS02235 (AM439_02230) 444955..445665 + 711 WP_017383381.1 outer membrane permeability protein SanA -
AM439_RS02240 (AM439_02235) 445800..447149 + 1350 WP_045333653.1 HipA domain-containing protein Toxin
AM439_RS02245 (AM439_02240) 447146..447583 + 438 WP_049134548.1 helix-turn-helix transcriptional regulator Antitoxin
AM439_RS02250 (AM439_02245) 447622..448632 - 1011 WP_003859318.1 galactose/methyl galactoside ABC transporter permease MglC -
AM439_RS02255 (AM439_02250) 448648..450168 - 1521 WP_003859320.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
AM439_RS02260 (AM439_02255) 450248..451246 - 999 WP_017383382.1 galactose/glucose ABC transporter substrate-binding protein MglB -
AM439_RS02265 (AM439_02260) 451542..452564 - 1023 WP_003859324.1 HTH-type transcriptional regulator GalS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51197.84 Da        Isoelectric Point: 4.6562

>T95539 WP_045333653.1 NZ_CP026719:445800-447149 [Enterobacter cloacae complex sp.]
METLTVQAYLNETWTDIALIKYPGSEKNDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRTLINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPTT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

>T95539 NZ_CP026719:445800-447149 [Enterobacter cloacae complex sp.]
ATGGAGACGTTAACCGTACAGGCATATCTGAACGAAACATGGACGGATATCGCTCTCATCAAATATCCAGGAAGTGAAAA
AAATGACTGGAATACCACGCAGTTAGACTATCTCACTGAGTATGCGATTAATTTTCTCGACTATGATGATTTTCACGCAG
TCTCAGTCAATCACCCTGTATCACTCTTTTTTGATGACCATGGCCAACCGGGCTGGCTGCGATTTATCGACGATATTATT
CCCGCCGGAGCAAGCCGCCGTTACTGGATTAATGCGCTCGACATCAGTGAATTACCCGTTGGCCAGCAAAATTTCTTATT
GCTGAAATTTGGCACAATGTCCCCTGTGGGGAATTTGCGCATAAAAGAATCCGTCCCAGAATGGAATAAATTCGCCAGCA
CTAAAACGTTTACGGTTGCGGACGTAATCGATCGTGCGGCCGATTTTCTGGATTATGCCCAGGAAAGAGGCGCGGCGGCG
GGTGGTGCAACCGGTGCTGGCGGTGAAGCGCCTAAACTTTTGCTGCGCTGTAGTGAAACTGATGCCATCTGGATCGATAC
CTGGCAAAACGAGCCGGATAACCGGGACCGCTATTATTTGGTTAAATATCCACGCGGTAACAGGACAGAAGTTGATTGTA
ATATTCTGCGAGCTGAATTCCATTATTATCATGAGCTTAACGCGATGGGTTTTTCCACGATCCCAGTGGAAACAATGCGC
CTTGAAGAAGGTAGCCATTACCCGTCTCTGTGGTTACCGCGTTTCGACATAGTGAGAAATGAGCAGGACCAACTGGTCAG
GCTGGGTATGGAATCGGTGTATTCGCTATTACAAAAGGCACCCGGCACCCTCCTTGATCATGAGACAACGTTGCGCACGT
TAATTAATAAAATCACCTCCAGCCATATGGTCCAGCAGCAGGGCTATCATTTTGATATTCCGGGATTTGTAACAGAATGG
GTGCGTCGCGATCTGCTAAATATTATTTTTGGAAATAGTGACAACCATGGGCGAAATACCGCCTTTGTGAAGGCTGAAAA
TCAAATTATGCTCTCCCCTGTATATGACTTTGCGCCGATGAAAGCCGATCCTGAAGGGATACCAAGAACCCTGAAGTGGT
CATTATCTTGCGAATCAGGAGGGGATTATAATTTCAACACTATCGCACAAACGCTGGCGGAGTGGATCCCTCCCACAACA
TTACTTGATGCTCTGCATGAAACAGCCGTTCAGCTTATTGATCTCCCGGAAAGGCTGGCGGCGCGAGGCGTTCCGGAACA
GATAATGGAAATGCCCGCGATGGGTTTTCGCTACATCCCGGATAAACTTTCCCGTTGGGGTTTATTATGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16113.57 Da        Isoelectric Point: 10.6379

>AT95539 WP_049134548.1 NZ_CP026719:447146-447583 [Enterobacter cloacae complex sp.]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEDPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGAALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp

>AT95539 NZ_CP026719:447146-447583 [Enterobacter cloacae complex sp.]
ATGAAAACACCTAATGAAGCTGAATCAGAGCTTTTACAGACATTAGCTCAAGTACGCAGCGTAAATAAAAAGCGACATCA
TGACGAGGCGGAAGATCCGGACAAGCCATCGGTAGTGAAGAGGCAACGCGTTACACAAGGCTTAACGCGGATTAGTACTC
TTGATCGCCAGGCTGTACTGCATGCAGCCATACGGGACATTTTGCTGGGGAAAATTACACAGGGAGCGGCGCTGAAAAGG
CTCAGGGTCGAGGTGTTAGGGCTGAAGCAGGATGAATATGCAAAGCTGGTCAGCGTATCCCGGAAAACATTGTCGGATGT
AGAAAATAACAGAGGCAATTATTCCGCTGACGTCATAAATAAAATCTTTAAACCCTTTGGGCTTCAAACCGGACTAGTGC
CCGTTTCAAAATCGCTTATCGCTTCACTTTTCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G5A822

Antitoxin

Source ID Structure

References