Detailed information of TA system TA095530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 64114..64850
• Replicon: plasmid unitig_3_pilon
• Accession: NZ_CP026718
• Organism: Klebsiella oxytoca strain AR_0028

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: AM355_RS30240
• Protein ID: WP_104877028.1
• Coordinates: 64114..64596 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AM355_RS30245
• Protein ID: WP_003026799.1
• Coordinates: 64584..64850 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM355_RS30220	59415..60218	-	804	WP_064407410.1	tetratricopeptide repeat protein	-
AM355_RS30225	60276..61622	-	1347	WP_077257948.1	ISNCY family transposase	-
AM355_RS30230	61849..62481	+	633	WP_001567369.1	hypothetical protein	-
AM355_RS30235	62510..63913	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
AM355_RS30240	64114..64596	-	483	WP_104877028.1	GNAT family N-acetyltransferase	Toxin
AM355_RS30245	64584..64850	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AM355_RS30250	65095..65529	-	435	WP_001567367.1	cell envelope integrity protein TolA	-
AM355_RS30255	65576..66124	-	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
AM355_RS30265	66618..66926	+	309	WP_017896554.1	hypothetical protein	-
AM355_RS30280	68151..68642	-	492	WP_004200903.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91175	91175

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17354.02 Da
• Isoelectric Point: 9.8720

>T95530 WP_104877028.1 NZ_CP026718:c64596-64114 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T95530 NZ_CP026718:c64596-64114 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT95530 WP_003026799.1 NZ_CP026718:c64850-64584 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT95530 NZ_CP026718:c64850-64584 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66