Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 129116..129852 Replicon plasmid unitig_1_pilon
Accession NZ_CP026716
Organism Klebsiella oxytoca strain AR_0028

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM355_RS29135 Protein ID WP_003026803.1
Coordinates 129370..129852 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM355_RS29130 Protein ID WP_003026799.1
Coordinates 129116..129382 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM355_RS29085 125178..125540 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
AM355_RS29090 125590..125940 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM355_RS29095 126298..126567 + 270 WP_004152102.1 hypothetical protein -
AM355_RS29100 126555..127130 + 576 WP_004152103.1 hypothetical protein -
AM355_RS29105 127161..127655 + 495 WP_004152104.1 DNA-binding protein -
AM355_RS29110 127699..128067 + 369 WP_004152105.1 hypothetical protein -
AM355_RS29115 128101..128304 + 204 WP_004152106.1 hemolysin expression modulator Hha -
AM355_RS29120 128353..128610 + 258 WP_004152107.1 hypothetical protein -
AM355_RS29125 128686..128940 + 255 WP_004152108.1 hypothetical protein -
AM355_RS29130 129116..129382 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM355_RS29135 129370..129852 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM355_RS29140 130053..131456 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
AM355_RS29145 131485..132117 - 633 WP_001567369.1 hypothetical protein -
AM355_RS29150 132343..133689 + 1347 WP_077253535.1 ISNCY family transposase -
AM355_RS29155 133738..134133 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..189585 189585


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T95529 WP_003026803.1 NZ_CP026716:129370-129852 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T95529 NZ_CP026716:129370-129852 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT95529 WP_003026799.1 NZ_CP026716:129116-129382 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT95529 NZ_CP026716:129116-129382 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References