Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4933660..4934207 Replicon chromosome
Accession NZ_CP026715
Organism Klebsiella oxytoca strain AR_0028

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag AM355_RS23695 Protein ID WP_014227299.1
Coordinates 4933660..4933968 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag AM355_RS23700 Protein ID WP_014227298.1
Coordinates 4933971..4934207 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM355_RS23665 4929307..4929618 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
AM355_RS23670 4929913..4930854 + 942 WP_014227303.1 LacI family transcriptional regulator -
AM355_RS23675 4930878..4931171 - 294 WP_014227302.1 hypothetical protein -
AM355_RS23680 4931381..4932346 + 966 WP_038423881.1 Rpn family recombination-promoting nuclease/putative transposase -
AM355_RS23685 4932346..4932603 + 258 WP_032718834.1 hypothetical protein -
AM355_RS23690 4932607..4933509 - 903 WP_014227300.1 EamA family transporter -
AM355_RS23695 4933660..4933968 - 309 WP_014227299.1 CcdB family protein Toxin
AM355_RS23700 4933971..4934207 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM355_RS23705 4934313..4935746 - 1434 WP_032718833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
AM355_RS23710 4935771..4936673 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
AM355_RS23715 4936834..4938000 - 1167 WP_085520169.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T95527 WP_014227299.1 NZ_CP026715:c4933968-4933660 [Klebsiella oxytoca]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T95527 NZ_CP026715:c4933968-4933660 [Klebsiella oxytoca]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGTATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT95527 WP_014227298.1 NZ_CP026715:c4934207-4933971 [Klebsiella oxytoca]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT95527 NZ_CP026715:c4934207-4933971 [Klebsiella oxytoca]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References