Detailed information of TA system TA095525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4152591..4153167
• Replicon: chromosome
• Accession: NZ_CP026715
• Organism: Klebsiella oxytoca strain AR_0028

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: AM355_RS19875
• Protein ID: WP_038424021.1
• Coordinates: 4152880..4153167 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: AM355_RS19870
• Protein ID: WP_014227757.1
• Coordinates: 4152591..4152893 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM355_RS19855	4149431..4149766	+	336	WP_085520253.1	endoribonuclease SymE	-
AM355_RS19860	4150219..4151130	+	912	WP_032719936.1	acetamidase/formamidase family protein	-
AM355_RS19865	4151127..4152470	+	1344	WP_049101852.1	APC family permease	-
AM355_RS19870	4152591..4152893	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
AM355_RS19875	4152880..4153167	-	288	WP_038424021.1	BrnT family toxin	Toxin
AM355_RS19880	4153418..4153861	-	444	WP_014227755.1	FosA family fosfomycin resistance glutathione transferase	-
AM355_RS19885	4153855..4154763	-	909	WP_085520252.1	LysR family transcriptional regulator	-
AM355_RS19890	4154851..4155633	+	783	WP_085520251.1	NAD(P)H-dependent oxidoreductase	-
AM355_RS19895	4155781..4156365	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
AM355_RS19900	4156511..4157311	+	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
AM355_RS19905	4157308..4157826	+	519	WP_004098240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11253.72 Da
• Isoelectric Point: 8.5899

>T95525 WP_038424021.1 NZ_CP026715:c4153167-4152880 [Klebsiella oxytoca]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T95525 NZ_CP026715:c4153167-4152880 [Klebsiella oxytoca]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT95525 WP_014227757.1 NZ_CP026715:c4152893-4152591 [Klebsiella oxytoca]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT95525 NZ_CP026715:c4152893-4152591 [Klebsiella oxytoca]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591