95523

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3450908..3451527 Replicon chromosome
Accession NZ_CP026715
Organism Klebsiella oxytoca strain AR_0028

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag AM355_RS16575 Protein ID WP_004099646.1
Coordinates 3451309..3451527 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag AM355_RS16570 Protein ID WP_004129911.1
Coordinates 3450908..3451282 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM355_RS16560 3446065..3447258 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM355_RS16565 3447281..3450427 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
AM355_RS16570 3450908..3451282 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
AM355_RS16575 3451309..3451527 + 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
AM355_RS16580 3451690..3452256 + 567 WP_038423299.1 maltose O-acetyltransferase -
AM355_RS16585 3452383..3452853 + 471 WP_014228205.1 YlaC family protein -
AM355_RS16590 3452828..3454282 - 1455 WP_014228204.1 PLP-dependent aminotransferase family protein -
AM355_RS16595 3454384..3455082 + 699 WP_014228203.1 GNAT family N-acetyltransferase -
AM355_RS16600 3455079..3455219 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM355_RS16605 3455219..3455482 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T95523 WP_004099646.1 NZ_CP026715:3451309-3451527 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T95523 NZ_CP026715:3451309-3451527 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT95523 WP_004129911.1 NZ_CP026715:3450908-3451282 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT95523 NZ_CP026715:3450908-3451282 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References