Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1561239..1561859 Replicon chromosome
Accession NZ_CP026709
Organism Citrobacter koseri strain AR_0024

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM351_RS07450 Protein ID WP_002892050.1
Coordinates 1561239..1561457 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag AM351_RS07455 Protein ID WP_012133514.1
Coordinates 1561485..1561859 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM351_RS07415 1556577..1557191 + 615 WP_012133522.1 membrane protein -
AM351_RS07420 1557271..1557624 + 354 WP_012133521.1 DUF1428 family protein -
AM351_RS07425 1557664..1559214 - 1551 WP_058668708.1 EAL domain-containing protein -
AM351_RS07430 1559440..1559700 + 261 WP_012133519.1 type B 50S ribosomal protein L31 -
AM351_RS07435 1559702..1559842 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM351_RS07440 1559920..1560390 - 471 WP_060815856.1 hypothetical protein -
AM351_RS07445 1560504..1561055 - 552 WP_049009805.1 maltose O-acetyltransferase -
AM351_RS07450 1561239..1561457 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM351_RS07455 1561485..1561859 - 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
AM351_RS07460 1562361..1565507 - 3147 WP_012133513.1 efflux RND transporter permease AcrB -
AM351_RS07465 1565530..1566723 - 1194 WP_047458420.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1550594..1561859 11265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95499 WP_002892050.1 NZ_CP026709:c1561457-1561239 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T95499 NZ_CP026709:c1561457-1561239 [Citrobacter koseri]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT95499 WP_012133514.1 NZ_CP026709:c1561859-1561485 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT95499 NZ_CP026709:c1561859-1561485 [Citrobacter koseri]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACCAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References