Detailed information of TA system TA095498

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1326095..1326798
• Replicon: chromosome
• Accession: NZ_CP026709
• Organism: Citrobacter koseri strain AR_0024

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: AM351_RS06315
• Protein ID: WP_058668643.1
• Coordinates: 1326433..1326798 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AM351_RS06310
• Protein ID: WP_058668642.1
• Coordinates: 1326095..1326412 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM351_RS06275	1321717..1321953	+	237	WP_001144029.1	hypothetical protein	-
AM351_RS06280	1321953..1322393	+	441	WP_058668635.1	hypothetical protein	-
AM351_RS06285	1322417..1322881	+	465	WP_058668636.1	hypothetical protein	-
AM351_RS06290	1323460..1324257	-	798	WP_125337065.1	hypothetical protein	-
AM351_RS24335	1324524..1324795	+	272	Protein_1167	DUF905 family protein	-
AM351_RS06295	1324898..1325356	+	459	WP_058668639.1	antirestriction protein	-
AM351_RS06300	1325365..1325847	+	483	WP_058668640.1	DNA repair protein RadC	-
AM351_RS06305	1325856..1326077	+	222	WP_058668641.1	DUF987 domain-containing protein	-
AM351_RS06310	1326095..1326412	+	318	WP_058668642.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM351_RS06315	1326433..1326798	+	366	WP_058668643.1	TA system toxin CbtA family protein	Toxin
AM351_RS06320	1327058..1328089	-	1032	WP_058668644.1	methionine synthase	-
AM351_RS06325	1328117..1329100	-	984	WP_058668645.1	DUF1852 domain-containing protein	-
AM351_RS06330	1329370..1330593	-	1224	WP_058668646.1	Zn-dependent hydrolase	-
AM351_RS06335	1330590..1331072	-	483	WP_012133757.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13610.76 Da
• Isoelectric Point: 8.2762

>T95498 WP_058668643.1 NZ_CP026709:1326433-1326798 [Citrobacter koseri]
MKTLPATISRAAKPCLSPVAVWQMLLARLLEKHYGLTLNDTPFFDESVIQEHIDAGITLANAVNFLVEKYELVRINRTGF
TSQEQAPYLTVTDILHARRACGLMNSCSYREVSNIVLSRSR

Nucleotide

• Length: 366 bp

>T95498 NZ_CP026709:1326433-1326798 [Citrobacter koseri]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCTCCAGTGGCTGTCTGGCAAATGTTACTGGC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTTTGATGAATCCGTTATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAAAAGTATGAGTTAGTACGTATCAATCGCACAGGATTT
ACCTCACAGGAACAAGCGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGCATGCGGATTAATGAACAGTTG
TTCGTATCGTGAAGTAAGTAATATTGTTCTTAGCCGATCGCGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11949.62 Da
• Isoelectric Point: 7.3230

>AT95498 WP_058668642.1 NZ_CP026709:1326095-1326412 [Citrobacter koseri]
MKNTTWGLQRDITPRLGARLMQEGNRLHYLADRASITGKFSDTECLKLDFAFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT95498 NZ_CP026709:1326095-1326412 [Citrobacter koseri]
ATGAAAAACACCACATGGGGCCTACAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGATGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCAAGTATCACCGGTAAGTTTAGTGATACCGAATGCCTGAAGTTGGATTTCGCATTCCCGC
ATTTTATCAGTCAGATGGAATCAATGCTGACTACTGGTGAAATGAACCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATATATCGCTGTTTATCCCACTCAACGCTAA