Detailed information of TA system TA095497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 420474..421090
• Replicon: chromosome
• Accession: NZ_CP026709
• Organism: Citrobacter koseri strain AR_0024

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A427P7X6
• Locus tag: AM351_RS01990
• Protein ID: WP_047459878.1
• Coordinates: 420716..421090 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AM351_RS01985
• Protein ID: WP_160880417.1
• Coordinates: 420474..420716 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM351_RS01965	417353..417952	+	600	WP_058668370.1	glucose-1-phosphatase	-
AM351_RS01970	417946..418818	+	873	WP_012133924.1	virulence factor BrkB family protein	-
AM351_RS01975	418815..419252	+	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM351_RS01980	419297..420238	+	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
AM351_RS01985	420474..420716	+	243	WP_160880417.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM351_RS01990	420716..421090	+	375	WP_047459878.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM351_RS01995	421127..422056	-	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
AM351_RS02000	422053..422688	-	636	WP_058668371.1	formate dehydrogenase cytochrome b556 subunit	-
AM351_RS02005	422685..423587	-	903	WP_012133916.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13799.04 Da
• Isoelectric Point: 9.4395

>T95497 WP_047459878.1 NZ_CP026709:420716-421090 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRDEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T95497 NZ_CP026709:420716-421090 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGATGAAGCGAAGAAGGCTCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATCGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTCATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTCGCGGGGATTCCGGGAGTCATTACGCCTTATCATTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9396.62 Da
• Isoelectric Point: 4.1961

>AT95497 WP_160880417.1 NZ_CP026709:420474-420716 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW

Nucleotide

• Length: 243 bp

>AT95497 NZ_CP026709:420474-420716 [Citrobacter koseri]
ATGATGGCTGGGATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTTAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAACTTCGCGAGGAGTGG
TGA

Structures

Toxin

• AlphaFold DB: A0A427P7X6