Detailed information of TA system TA095489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4655235..4655797
• Replicon: chromosome
• Accession: NZ_CP026702
• Organism: Serratia marcescens strain AR_0027

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A7D5Y033
• Locus tag: AM354_RS22210
• Protein ID: WP_033655000.1
• Coordinates: 4655441..4655797 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A8G2G5I1
• Locus tag: AM354_RS22205
• Protein ID: WP_004936539.1
• Coordinates: 4655235..4655444 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM354_RS22185	4651385..4651711	+	327	WP_004936551.1	phage holin, lambda family	-
AM354_RS22190	4651701..4652102	+	402	WP_021505638.1	M15 family metallopeptidase	-
AM354_RS22195	4652099..4652479	+	381	WP_033635477.1	DUF2570 family protein	-
AM354_RS26585	4652460..4652633	+	174	WP_033635476.1	hypothetical protein	-
AM354_RS22200	4652846..4655125	+	2280	WP_016926766.1	NADP-dependent oxaloacetate-decarboxylating malate dehydrogenase	-
AM354_RS22205	4655235..4655444	+	210	WP_004936539.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
AM354_RS22210	4655441..4655797	+	357	WP_033655000.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AM354_RS22215	4655808..4656746	-	939	WP_049233579.1	homocysteine S-methyltransferase	-
AM354_RS22220	4656736..4658145	-	1410	WP_033654998.1	S-methylmethionine permease	-
AM354_RS22225	4658323..4659162	+	840	WP_041038076.1	ChaN family lipoprotein	-
AM354_RS22230	4659265..4660068	+	804	WP_033649853.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (20-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13086.82 Da
• Isoelectric Point: 4.5654

>T95489 WP_033655000.1 NZ_CP026702:4655441-4655797 [Serratia marcescens]
VIFLTAEDIAEFNAEIVPHGRQDGSKVEAVANRVLNAYHYENVTDVYRLAALYLIAISHGHIFLDGNKRTAFQSMALFLG
INGVALREDAELVEITVEAAQGRLNVEQAAEYLRLLTE

Nucleotide

• Length: 357 bp

>T95489 NZ_CP026702:4655441-4655797 [Serratia marcescens]
GTGATCTTTTTGACCGCAGAGGATATTGCGGAATTCAATGCTGAAATTGTGCCTCACGGCCGCCAGGATGGCAGCAAAGT
TGAGGCTGTAGCCAACCGCGTGCTCAATGCTTACCACTACGAGAACGTCACTGACGTTTACCGTTTGGCCGCGCTCTATC
TCATCGCCATCAGTCACGGTCACATTTTTCTTGATGGCAACAAACGCACGGCCTTCCAAAGCATGGCGCTATTTCTCGGT
ATTAATGGCGTTGCACTGCGGGAAGACGCAGAACTGGTCGAAATTACGGTTGAAGCAGCTCAAGGCCGCCTCAACGTTGA
ACAAGCCGCTGAATACTTACGCCTGCTAACCGAATAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7749.96 Da
• Isoelectric Point: 10.1768

>AT95489 WP_004936539.1 NZ_CP026702:4655235-4655444 [Serratia marcescens]
MTTISYTAARNNLAEVLLEAQKQPVEVTRRGHDEVYIISKADYEVLVKAKVKARIQAKHADTIKALANR

Nucleotide

• Length: 210 bp

>AT95489 NZ_CP026702:4655235-4655444 [Serratia marcescens]
ATGACTACGATTAGCTACACGGCGGCGAGAAACAATTTGGCCGAGGTGCTTTTGGAAGCGCAAAAACAGCCGGTGGAAGT
GACGCGCCGCGGACACGATGAGGTTTACATCATCAGTAAGGCCGACTATGAGGTTTTGGTCAAAGCCAAGGTGAAAGCGC
GTATTCAGGCTAAACACGCGGATACGATTAAGGCGCTGGCGAATAGGTGA

Structures

Toxin

• AlphaFold DB: A0A7D5Y033