Detailed information of TA system TA095472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 74041..74566
• Replicon: plasmid unitig_1_pilon
• Accession: NZ_CP026701
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain AR_0031

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: AM358_RS25485
• Protein ID: WP_001159863.1
• Coordinates: 74261..74566 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: AM358_RS25480
• Protein ID: WP_000813641.1
• Coordinates: 74041..74259 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM358_RS25450	69328..69709	+	382	Protein_82	YgiW/YdeI family stress tolerance OB fold protein	-
AM358_RS25455	69878..70322	+	445	Protein_83	site-specific integrase	-
AM358_RS25460	70581..71570	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
AM358_RS25465	72064..72360	-	297	WP_001687482.1	hypothetical protein	-
AM358_RS25475	72844..73365	-	522	WP_010999942.1	hypothetical protein	-
AM358_RS25480	74041..74259	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
AM358_RS25485	74261..74566	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
AM358_RS25490	74568..74858	+	291	WP_001266176.1	hypothetical protein	-
AM358_RS25495	74855..75376	+	522	WP_000198608.1	hypothetical protein	-
AM358_RS25500	75411..76193	+	783	WP_000082169.1	site-specific integrase	-
AM358_RS25505	76202..76915	+	714	Protein_92	EAL domain-containing protein	-
AM358_RS25515	76919..77428	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
AM358_RS25520	77422..77907	+	486	WP_000905606.1	membrane protein	-
AM358_RS25720	78184..78471	-	288	WP_071530243.1	hypothetical protein	-
AM358_RS25525	78627..79187	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93832	93832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T95472 WP_001159863.1 NZ_CP026701:74261-74566 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T95472 NZ_CP026701:74261-74566 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT95472 WP_000813641.1 NZ_CP026701:74041-74259 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT95472 NZ_CP026701:74041-74259 [Salmonella enterica subsp. enterica serovar Typhimurium]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6