95468

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4390441..4391061 Replicon chromosome
Accession NZ_CP026697
Organism Citrobacter koseri strain AR_0025

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AM352_RS21585 Protein ID WP_002892050.1
Coordinates 4390843..4391061 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag AM352_RS21580 Protein ID WP_012133514.1
Coordinates 4390441..4390815 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM352_RS21570 4385577..4386770 + 1194 WP_047458420.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM352_RS21575 4386793..4389939 + 3147 WP_012133513.1 efflux RND transporter permease AcrB -
AM352_RS21580 4390441..4390815 + 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
AM352_RS21585 4390843..4391061 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AM352_RS21590 4391245..4391796 + 552 WP_049009805.1 maltose O-acetyltransferase -
AM352_RS21595 4391910..4392380 + 471 WP_024130587.1 membrane protein -
AM352_RS21600 4392458..4392598 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
AM352_RS21605 4392600..4392860 - 261 WP_012133519.1 type B 50S ribosomal protein L31 -
AM352_RS21610 4393086..4394636 + 1551 WP_058668708.1 EAL domain-containing protein -
AM352_RS21615 4394676..4395029 - 354 WP_012133521.1 DUF1428 family protein -
AM352_RS21620 4395109..4395723 - 615 WP_104868983.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4390441..4398485 8044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95468 WP_002892050.1 NZ_CP026697:4390843-4391061 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T95468 NZ_CP026697:4390843-4391061 [Citrobacter koseri]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT95468 WP_012133514.1 NZ_CP026697:4390441-4390815 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT95468 NZ_CP026697:4390441-4390815 [Citrobacter koseri]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACAAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References