Detailed information of TA system TA095456

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 1957470..1958188
• Replicon: chromosome
• Accession: NZ_CP026697
• Organism: Citrobacter koseri strain AR_0025

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: AM352_RS09555
• Protein ID: WP_104868302.1
• Coordinates: 1957470..1957799 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AM352_RS09560
• Protein ID: WP_104868303.1
• Coordinates: 1957847..1958188 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM352_RS09525	1952716..1953405	-	690	WP_012134736.1	acireductone synthase	-
AM352_RS09530	1953402..1954016	-	615	WP_104868297.1	methylthioribulose 1-phosphate dehydratase	-
AM352_RS09535	1954607..1955221	-	615	WP_104868298.1	YagU family protein	-
AM352_RS09540	1955579..1955818	-	240	WP_104868299.1	hypothetical protein	-
AM352_RS09545	1955815..1956054	-	240	WP_104868300.1	DinI family protein	-
AM352_RS09550	1956306..1957088	-	783	WP_104868301.1	toll/interleukin-1 receptor domain-containing protein	-
AM352_RS09555	1957470..1957799	-	330	WP_104868302.1	TA system toxin CbtA family protein	Toxin
AM352_RS09560	1957847..1958188	-	342	WP_104868303.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM352_RS09565	1958211..1958432	-	222	WP_104868304.1	DUF987 domain-containing protein	-
AM352_RS09570	1958416..1958925	-	510	WP_104868305.1	DNA repair protein RadC	-
AM352_RS09575	1958937..1959332	-	396	WP_181397040.1	antirestriction protein	-
AM352_RS09585	1959665..1960486	-	822	WP_167594645.1	DUF945 domain-containing protein	-
AM352_RS09590	1960566..1960907	-	342	WP_104868308.1	hypothetical protein	-
AM352_RS09595	1960928..1961392	-	465	WP_104868309.1	hypothetical protein	-
AM352_RS24375	1961421..1961675	-	255	WP_181397041.1	hypothetical protein	-
AM352_RS09605	1961887..1962774	-	888	WP_104868310.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1955579..1986451	30872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (12-112)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12407.16 Da
• Isoelectric Point: 8.9981

>T95456 WP_104868302.1 NZ_CP026697:c1957799-1957470 [Citrobacter koseri]
MQTLSSHPIRATQPCLSPVETWQRLLTHLLSQHYGLTLNDTPFSNETTIREHIDAGVSLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYLSVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T95456 NZ_CP026697:c1957799-1957470 [Citrobacter koseri]
ATGCAAACCTTATCATCACACCCGATACGGGCGACTCAGCCCTGTCTGTCACCCGTTGAAACCTGGCAACGGCTGCTGAC
CCATCTGTTATCGCAGCACTATGGCCTAACGCTGAATGACACACCGTTCAGTAATGAAACCACCATACGGGAACATATTG
ATGCTGGAGTATCGCTAAGCGATGCGGTGAACTTTCTGGTAGAAAAATACGGGCTTGTCCGTATCGATCGGAAAGGATTT
TCGTGGCAAGAGCAGACCCCGTATCTTTCCGTAGTGGATATTTTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12703.55 Da
• Isoelectric Point: 6.8312

>AT95456 WP_104868303.1 NZ_CP026697:c1958188-1957847 [Citrobacter koseri]
MKNIKNHDIAGPWWGLKPTVMPCFGVRLVQEGNRLHYLADRASITGSFSDADLRHLDQAFPVLLKQLELMLVSGELTSHH
QHRVTLYHNGLTCEADTLGSCGYVYIAIYPDQP

Nucleotide

• Length: 342 bp

>AT95456 NZ_CP026697:c1958188-1957847 [Citrobacter koseri]
ATGAAAAACATCAAAAATCATGATATTGCTGGCCCCTGGTGGGGACTTAAACCCACCGTCATGCCATGCTTTGGCGTACG
ACTGGTACAGGAAGGCAACCGGCTGCATTATCTGGCTGACCGGGCCAGTATCACCGGGTCATTCAGCGACGCGGATTTAC
GCCATCTCGACCAGGCGTTTCCGGTGCTGCTTAAACAGCTGGAGTTGATGCTCGTTTCCGGTGAGCTCACCTCCCACCAT
CAGCACCGCGTCACGCTATATCACAATGGACTGACTTGCGAAGCGGATACACTCGGCTCCTGTGGTTATGTCTACATCGC
TATTTATCCCGATCAGCCTTAA