Detailed information of TA system TA095453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 673081..673697
• Replicon: chromosome
• Accession: NZ_CP026697
• Organism: Citrobacter koseri strain AR_0025

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: AM352_RS03165
• Protein ID: WP_012133919.1
• Coordinates: 673081..673455 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AM352_RS03170
• Protein ID: WP_160880417.1
• Coordinates: 673455..673697 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM352_RS03150	670584..671486	+	903	WP_012133916.1	formate dehydrogenase O subunit beta	-
AM352_RS03155	671483..672118	+	636	WP_049011887.1	formate dehydrogenase cytochrome b556 subunit	-
AM352_RS03160	672115..673044	+	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
AM352_RS03165	673081..673455	-	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM352_RS03170	673455..673697	-	243	WP_160880417.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM352_RS03175	673933..674874	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
AM352_RS03180	674919..675356	-	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AM352_RS03185	675353..676225	-	873	WP_012133924.1	virulence factor BrkB family protein	-
AM352_RS03190	676219..676818	-	600	WP_104867942.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T95453 WP_012133919.1 NZ_CP026697:c673455-673081 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T95453 NZ_CP026697:c673455-673081 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGGTGAAGCGAAGAAGGCTCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAATACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATCGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTTATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTCGCGGGGATTCCGGGAGTCATTACGCCTTACCATTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9396.62 Da
• Isoelectric Point: 4.1961

>AT95453 WP_160880417.1 NZ_CP026697:c673697-673455 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW

Nucleotide

• Length: 243 bp

>AT95453 NZ_CP026697:c673697-673455 [Citrobacter koseri]
ATGATGGCTGGAATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTCAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAACTTCGCGAGGAGTGG
TGA

Structures

Toxin

• AlphaFold DB: A8AL49