Detailed information of TA system TA095435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 5229084..5230062
• Replicon: chromosome
• Accession: NZ_CP026678
• Organism: Bacillus cereus strain TG1-6

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: M1Q940
• Locus tag: C2I25_RS27545
• Protein ID: WP_000624982.1
• Coordinates: 5229325..5230062 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: C2I25_RS27540
• Protein ID: WP_000237818.1
• Coordinates: 5229084..5229212 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2I25_RS27510	5225740..5226129	+	390	WP_106825070.1	YxeA family protein	-
C2I25_RS27515	5226147..5226323	-	177	Protein_5135	stage II sporulation protein SB	-
C2I25_RS27520	5226653..5227633	+	981	WP_106825071.1	WXG100 family type VII secretion target	-
C2I25_RS29025	5227694..5228488	+	795	WP_176470977.1	hypothetical protein	-
C2I25_RS27530	5228515..5228790	+	276	WP_063547286.1	hypothetical protein	-
C2I25_RS29030	5228835..5229011	-	177	WP_000852631.1	hypothetical protein	-
C2I25_RS27540	5229084..5229212	-	129	WP_000237818.1	hypothetical protein	Antitoxin
C2I25_RS27545	5229325..5230062	-	738	WP_000624982.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
C2I25_RS27555	5230301..5231038	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
C2I25_RS27560	5231205..5231687	-	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
C2I25_RS27565	5231795..5233480	+	1686	WP_189340001.1	alpha-keto acid decarboxylase family protein	-
C2I25_RS27570	5233638..5234420	-	783	Protein_5145	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28323.03 Da
• Isoelectric Point: 8.2998

>T95435 WP_000624982.1 NZ_CP026678:c5230062-5229325 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T95435 NZ_CP026678:c5230062-5229325 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACTGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTTGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT95435 WP_000237818.1 NZ_CP026678:c5229212-5229084 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT95435 NZ_CP026678:c5229212-5229084 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: M1Q940

Antitoxin

• AlphaFold DB: W8YZL5