Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1979668..1980310 Replicon chromosome
Accession NZ_CP026678
Organism Bacillus cereus strain TG1-6

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag C2I25_RS10255 Protein ID WP_000635965.1
Coordinates 1979668..1980018 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag C2I25_RS10260 Protein ID WP_000004570.1
Coordinates 1980023..1980310 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2I25_RS10240 1976603..1977061 - 459 WP_071713820.1 SprT family protein -
C2I25_RS10245 1977257..1977373 + 117 WP_001143640.1 cortex morphogenetic protein CmpA -
C2I25_RS10250 1977431..1979599 - 2169 WP_145959718.1 RNA-binding transcriptional accessory protein -
C2I25_RS10255 1979668..1980018 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C2I25_RS10260 1980023..1980310 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
C2I25_RS10265 1980621..1981790 - 1170 WP_000390593.1 alanine racemase -
C2I25_RS10270 1981908..1982858 - 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
C2I25_RS10275 1983015..1983374 - 360 WP_053565408.1 holo-ACP synthase -
C2I25_RS10280 1983468..1984040 + 573 WP_106823337.1 rhomboid family intramembrane serine protease -
C2I25_RS10285 1984033..1984995 - 963 WP_106823339.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T95434 WP_000635965.1 NZ_CP026678:c1980018-1979668 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T95434 NZ_CP026678:c1980018-1979668 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT95434 WP_000004570.1 NZ_CP026678:c1980310-1980023 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT95434 NZ_CP026678:c1980310-1980023 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References