Detailed information of TA system TA095422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3801338..3802257
• Replicon: chromosome
• Accession: NZ_CP026673
• Organism: Bacillus licheniformis strain 14ADL4

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: BL14DL4_RS19930
• Protein ID: WP_003180879.1
• Coordinates: 3801511..3802257 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: Q65L00
• Locus tag: BL14DL4_RS19925
• Protein ID: WP_011197812.1
• Coordinates: 3801338..3801511 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BL14DL4_RS19905	3796763..3797860	+	1098	WP_003180867.1	mannonate dehydratase	-
BL14DL4_RS19910	3797836..3798684	+	849	WP_003180869.1	SDR family oxidoreductase	-
BL14DL4_RS19915	3798711..3799724	+	1014	WP_011197810.1	zinc-binding alcohol dehydrogenase family protein	-
BL14DL4_RS19920	3799777..3801048	+	1272	WP_011197811.1	MFS transporter	-
BL14DL4_RS19925	3801338..3801511	-	174	WP_011197812.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BL14DL4_RS19930	3801511..3802257	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BL14DL4_RS19935	3802368..3803366	-	999	WP_009328723.1	inorganic phosphate transporter	-
BL14DL4_RS19940	3803379..3803996	-	618	WP_003180884.1	DUF47 domain-containing protein	-
BL14DL4_RS19945	3804328..3806085	+	1758	WP_095266789.1	gamma-glutamyltransferase	-
BL14DL4_RS19950	3806222..3806866	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T95422 WP_003180879.1 NZ_CP026673:c3802257-3801511 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T95422 NZ_CP026673:c3802257-3801511 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6971.08 Da
• Isoelectric Point: 10.7815

>AT95422 WP_011197812.1 NZ_CP026673:c3801511-3801338 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPYTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT95422 NZ_CP026673:c3801511-3801338 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCTATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: Q65L00