Detailed information of TA system TA095418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 479830..480395
• Replicon: chromosome
• Accession: NZ_CP026671
• Organism: Leptospira borgpetersenii serovar Ceylonica strain Piyasena

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V6I350
• Locus tag: C4Q31_RS02255
• Protein ID: WP_002753850.1
• Coordinates: 479830..480078 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C4Q31_RS02260
• Protein ID: WP_011670965.1
• Coordinates: 480072..480395 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4Q31_RS02230	474878..475879	+	1002	WP_002732926.1	metallophosphoesterase	-
C4Q31_RS02235	475882..476559	+	678	WP_002725755.1	hypothetical protein	-
C4Q31_RS02240	476556..476972	+	417	WP_118883418.1	hypothetical protein	-
C4Q31_RS02245	476953..479133	+	2181	WP_002732919.1	ATP-dependent DNA helicase RecG	-
C4Q31_RS02255	479830..480078	+	249	WP_002753850.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
C4Q31_RS02260	480072..480395	+	324	WP_011670965.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
C4Q31_RS02265	480831..482366	+	1536	WP_002738394.1	phosphomethylpyrimidine synthase ThiC	-
C4Q31_RS02270	482706..483398	+	693	WP_002738931.1	helicase	-
C4Q31_RS02275	483773..484117	-	345	WP_002728323.1	TIGR04452 family lipoprotein	-
C4Q31_RS02285	484450..485193	+	744	WP_002728313.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (6-49)

Antitoxin

• mRNA interferase PemK-like (5-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9459.00 Da
• Isoelectric Point: 5.1749

>T95418 WP_002753850.1 NZ_CP026671:479830-480078 [Leptospira borgpetersenii serovar Ceylonica]
MKALVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKSMSSNKDDKLLIPDSIDLSDKDW
EW

Nucleotide

• Length: 249 bp

>T95418 NZ_CP026671:479830-480078 [Leptospira borgpetersenii serovar Ceylonica]
ATGAAGGCTTTAGTTGTAAAAATAGGTAATTCAAAAGGAATTCGGATTCCCAAAGCGGTTTTAGAAGAATGCCATATTGA
AGAAGAAGTAGATTTACTAATCGATAACAATAAATTAATTATTGTGCCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAATCAATGTCATCTAATAAAGATGATAAATTGCTTATTCCTGATTCAATAGATTTATCGGATAAGGATTGG
GAATGGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12296.80 Da
• Isoelectric Point: 10.5161

>AT95418 WP_011670965.1 NZ_CP026671:480072-480395 [Leptospira borgpetersenii serovar Ceylonica]
MVISQYEVYLINLDPTVGHEIKKSRPCVIISPNEMNKTIGTIIITPMTTKSRSYPTRVELTFQGKKGWIVLDQIRTVDKI
RLIKKLGKIDPKTVNKMKLIIKEMLVD

Nucleotide

• Length: 324 bp

>AT95418 NZ_CP026671:480072-480395 [Leptospira borgpetersenii serovar Ceylonica]
ATGGTGATTTCTCAATACGAAGTATATTTGATAAATTTAGATCCAACGGTCGGACATGAGATTAAAAAATCAAGACCTTG
CGTAATAATCTCACCTAATGAAATGAATAAAACTATCGGAACAATTATTATTACACCAATGACTACAAAATCTCGATCAT
ATCCAACAAGAGTCGAATTAACATTTCAAGGAAAGAAAGGCTGGATTGTGTTAGATCAAATTCGAACTGTAGATAAAATA
CGTCTGATAAAAAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGATTATAAAGGAAATGTTGGTTGA
TTAA

Structures

Toxin

• AlphaFold DB: V6I350