Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1749986..1750654 Replicon chromosome
Accession NZ_CP026670
Organism Streptococcus pneumoniae strain endophthalmitis isolate 335

Toxin (Protein)


Gene name hicA Uniprot ID I0T4C6
Locus tag C4N11_RS09215 Protein ID WP_001132287.1
Coordinates 1750475..1750654 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag C4N11_RS09210 Protein ID WP_000961810.1
Coordinates 1749986..1750438 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4N11_RS09190 1746835..1747869 - 1035 WP_000747915.1 ATP-binding protein -
C4N11_RS09195 1747979..1748137 - 159 WP_000418159.1 7,8-dihydro-8-oxoguanine-triphosphatase -
C4N11_RS09200 1748118..1749206 - 1089 WP_000719700.1 M50 family metallopeptidase -
C4N11_RS09205 1749225..1749695 - 471 WP_000257084.1 DUF3013 family protein -
C4N11_RS09210 1749986..1750438 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C4N11_RS09215 1750475..1750654 - 180 WP_001132287.1 type II toxin-antitoxin system HicA family toxin Toxin
C4N11_RS09220 1750791..1750970 - 180 WP_001048906.1 hypothetical protein -
C4N11_RS09230 1751135..1751374 - 240 WP_000818080.1 hypothetical protein -
C4N11_RS09240 1751644..1752915 + 1272 WP_001113205.1 replication-associated recombination protein A -
C4N11_RS09250 1753460..1754779 - 1320 WP_000502535.1 glycoside hydrolase family 32 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6697.91 Da        Isoelectric Point: 11.0174

>T95417 WP_001132287.1 NZ_CP026670:c1750654-1750475 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T95417 NZ_CP026670:c1750654-1750475 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGTTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACCTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT95417 WP_000961810.1 NZ_CP026670:c1750438-1749986 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT95417 NZ_CP026670:c1750438-1749986 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I0T4C6

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References