Detailed information of TA system TA095416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1230604..1231838
• Replicon: chromosome
• Accession: NZ_CP026670
• Organism: Streptococcus pneumoniae strain endophthalmitis isolate 335

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: B2IQC7
• Locus tag: C4N11_RS06490
• Protein ID: WP_000512672.1
• Coordinates: 1230604..1231362 (-)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: C4N11_RS06495
• Protein ID: WP_000579607.1
• Coordinates: 1231362..1231838 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4N11_RS06465	1226244..1227167	+	924	WP_000417606.1	protein rep	-
C4N11_RS06475	1227963..1228682	-	720	Protein_1214	type A chloramphenicol O-acetyltransferase	-
C4N11_RS06480	1228853..1229101	-	249	WP_001814254.1	hypothetical protein	-
C4N11_RS06485	1229340..1230128	-	789	WP_000214003.1	hypothetical protein	-
C4N11_RS06490	1230604..1231362	-	759	WP_000512672.1	zeta toxin family protein	Toxin
C4N11_RS06495	1231362..1231838	-	477	WP_000579607.1	helix-turn-helix domain-containing protein	Antitoxin
C4N11_RS06500	1231908..1232180	-	273	WP_000494445.1	hypothetical protein	-
C4N11_RS06505	1232218..1232601	-	384	WP_000159218.1	hypothetical protein	-
C4N11_RS06510	1232598..1232831	-	234	WP_000152419.1	hypothetical protein	-
C4N11_RS06515	1232882..1233967	-	1086	WP_000401892.1	toprim domain-containing protein	-
C4N11_RS06520	1233977..1234618	-	642	WP_000933090.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	cat(pC194)	-	1192476..1264623	72147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (24-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28861.84 Da
• Isoelectric Point: 8.6386

>T95416 WP_000512672.1 NZ_CP026670:c1231362-1230604 [Streptococcus pneumoniae]
MGIQDYTDSQFKQALARNIRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKYFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEIQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRGCVYDAKENTTSAADVLQELLFGEWSQVEKDML
KSGEERLKDLTN

Nucleotide

• Length: 759 bp

>T95416 NZ_CP026670:c1231362-1230604 [Streptococcus pneumoniae]
ATGGGAATCCAAGATTATACCGATAGTCAATTTAAACAGGCTTTAGCAAGGAACATTCGCTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTCGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTTCAGCAA
GAATATGGCAAAGACAGTGTTGAATACACCAAATATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAAGAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACTATTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAGTA
GGGGATATGAAATACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTGTACGCTATTAACCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAGGTTGTGTATATGATGCAA
AAGAAAATACAACTTCAGCGGCAGATGTTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAAGTAGAGAAGGATATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACTAATTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18279.74 Da
• Isoelectric Point: 4.4188

>AT95416 WP_000579607.1 NZ_CP026670:c1231838-1231362 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEIFEKRMAA

Nucleotide

• Length: 477 bp

>AT95416 NZ_CP026670:c1231838-1231362 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTGCGTAAAACACATGACTTAACACAACTCGAATTTGCACGGATTGTAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATTTGAAAAACGGATGGCAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A4J1Y0E8