Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4241290..4241787 Replicon chromosome
Accession NZ_CP026641
Organism Escherichia coli strain FORC_082

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FORC82_RS21130 Protein ID WP_001173343.1
Coordinates 4241290..4241463 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FORC82_RS21135 Protein ID WP_000398619.1
Coordinates 4241491..4241787 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC82_RS21120 (4237960) 4237960..4239417 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
FORC82_RS21125 (4239654) 4239654..4241171 + 1518 WP_001295090.1 lysine--tRNA ligase -
FORC82_RS21130 (4241290) 4241290..4241463 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FORC82_RS21135 (4241491) 4241491..4241787 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FORC82_RS21140 (4242014) 4242014..4242286 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
FORC82_RS21145 (4242298) 4242298..4242528 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
FORC82_RS21150 (4242709) 4242709..4244340 + 1632 WP_001216477.1 sensor histidine kinase -
FORC82_RS21155 (4244337) 4244337..4245056 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T95361 WP_001173343.1 NZ_CP026641:c4241463-4241290 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T95361 NZ_CP026641:c4241463-4241290 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT95361 WP_000398619.1 NZ_CP026641:c4241787-4241491 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT95361 NZ_CP026641:c4241787-4241491 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References