Detailed information of TA system TA095360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4047295..4047871
• Replicon: chromosome
• Accession: NZ_CP026641
• Organism: Escherichia coli strain FORC_082

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: FORC82_RS20145
• Protein ID: WP_001297643.1
• Coordinates: 4047584..4047871 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: FORC82_RS20140
• Protein ID: WP_096969605.1
• Coordinates: 4047295..4047597 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC82_RS20125 (4043891)	4043891..4046041	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
FORC82_RS20130 (4046091)	4046091..4046294	+	204	WP_000467859.1	YbdD/YjiX family protein	-
FORC82_RS20135 (4046305)	4046305..4047261	+	957	WP_001297640.1	GTPase	-
FORC82_RS20140 (4047295)	4047295..4047597	-	303	WP_096969605.1	BrnA antitoxin family protein	Antitoxin
FORC82_RS20145 (4047584)	4047584..4047871	-	288	WP_001297643.1	BrnT family toxin	Toxin
FORC82_RS20150 (4048070)	4048070..4048767	-	698	Protein_3927	IS1 family transposase	-
FORC82_RS20155 (4048824)	4048824..4048919	+	96	Protein_3928	dienelactone hydrolase family protein	-
FORC82_RS20160 (4049065)	4049065..4050297	+	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
FORC82_RS20165 (4050338)	4050338..4051627	+	1290	WP_129501561.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T95360 WP_001297643.1 NZ_CP026641:c4047871-4047584 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T95360 NZ_CP026641:c4047871-4047584 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11493.93 Da
• Isoelectric Point: 8.4751

>AT95360 WP_096969605.1 NZ_CP026641:c4047597-4047295 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYQPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT95360 NZ_CP026641:c4047597-4047295 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCAGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9