Detailed information of TA system TA095357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3526752..3527589
• Replicon: chromosome
• Accession: NZ_CP026641
• Organism: Escherichia coli strain FORC_082

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A140NB16
• Locus tag: FORC82_RS17520
• Protein ID: WP_000227786.1
• Coordinates: 3527047..3527589 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FORC82_RS17515
• Protein ID: WP_001297137.1
• Coordinates: 3526752..3527063 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC82_RS17490 (3521772)	3521772..3522719	+	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-
FORC82_RS17495 (3522741)	3522741..3524732	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FORC82_RS17500 (3524722)	3524722..3525336	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FORC82_RS17505 (3525336)	3525336..3525665	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FORC82_RS17510 (3525677)	3525677..3526567	+	891	WP_000971336.1	heme o synthase	-
FORC82_RS17515 (3526752)	3526752..3527063	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FORC82_RS17520 (3527047)	3527047..3527589	+	543	WP_000227786.1	GNAT family N-acetyltransferase	Toxin
FORC82_RS17525 (3527645)	3527645..3528580	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
FORC82_RS17530 (3528997)	3528997..3530361	+	1365	WP_001000978.1	MFS transporter	-
FORC82_RS17535 (3530489)	3530489..3530980	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
FORC82_RS17540 (3531148)	3531148..3532059	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T95357 WP_000227786.1 NZ_CP026641:3527047-3527589 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T95357 NZ_CP026641:3527047-3527589 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT95357 WP_001297137.1 NZ_CP026641:3526752-3527063 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT95357 NZ_CP026641:3526752-3527063 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A140NB16

Antitoxin

• AlphaFold DB: A0A0E0Y6B3