Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3492660..3493278 Replicon chromosome
Accession NZ_CP026641
Organism Escherichia coli strain FORC_082

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FORC82_RS17350 Protein ID WP_001291435.1
Coordinates 3493060..3493278 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FORC82_RS17345 Protein ID WP_000344800.1
Coordinates 3492660..3493034 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC82_RS17335 (3487749) 3487749..3488942 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FORC82_RS17340 (3488965) 3488965..3492114 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FORC82_RS17345 (3492660) 3492660..3493034 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FORC82_RS17350 (3493060) 3493060..3493278 + 219 WP_001291435.1 HHA domain-containing protein Toxin
FORC82_RS17355 (3493450) 3493450..3494001 + 552 WP_000102564.1 maltose O-acetyltransferase -
FORC82_RS17360 (3494117) 3494117..3494587 + 471 WP_000136192.1 YlaC family protein -
FORC82_RS17365 (3494751) 3494751..3496301 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FORC82_RS17370 (3496343) 3496343..3496696 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FORC82_RS17380 (3497075) 3497075..3497386 + 312 WP_000409911.1 MGMT family protein -
FORC82_RS17385 (3497417) 3497417..3497989 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95356 WP_001291435.1 NZ_CP026641:3493060-3493278 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T95356 NZ_CP026641:3493060-3493278 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT95356 WP_000344800.1 NZ_CP026641:3492660-3493034 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT95356 NZ_CP026641:3492660-3493034 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References