Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1057809..1058392 Replicon chromosome
Accession NZ_CP026641
Organism Escherichia coli strain FORC_082

Toxin (Protein)


Gene name mazF Uniprot ID A0A1V3W215
Locus tag FORC82_RS05085 Protein ID WP_000254741.1
Coordinates 1058057..1058392 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FORC82_RS05080 Protein ID WP_000581937.1
Coordinates 1057809..1058057 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC82_RS05070 (1054148) 1054148..1055449 + 1302 WP_129501461.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
FORC82_RS05075 (1055497) 1055497..1057731 + 2235 WP_000226815.1 GTP pyrophosphokinase -
FORC82_RS05080 (1057809) 1057809..1058057 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FORC82_RS05085 (1058057) 1058057..1058392 + 336 WP_000254741.1 endoribonuclease MazF Toxin
FORC82_RS05090 (1058463) 1058463..1059254 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
FORC82_RS05095 (1059482) 1059482..1061119 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FORC82_RS05100 (1061207) 1061207..1062505 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12114.04 Da        Isoelectric Point: 8.2618

>T95344 WP_000254741.1 NZ_CP026641:1058057-1058392 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGSTKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T95344 NZ_CP026641:1058057-1058392 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGATCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT95344 WP_000581937.1 NZ_CP026641:1057809-1058057 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT95344 NZ_CP026641:1057809-1058057 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1V3W215

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References