Detailed information of TA system TA095305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 5464..6048
• Replicon: plasmid pSWBY1
• Accession: NZ_CP026617
• Organism: Acinetobacter sp. SWBY1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: C3Y93_RS14085
• Protein ID: WP_000286964.1
• Coordinates: 5746..6048 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C3Y93_RS14080
• Protein ID: WP_000985609.1
• Coordinates: 5464..5745 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3Y93_RS14050	526..1137	+	612	WP_044432563.1	recombinase family protein	-
C3Y93_RS14055	1327..2211	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C3Y93_RS14060	2267..3742	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C3Y93_RS14065	4235..4507	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C3Y93_RS14070	4500..4820	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C3Y93_RS14075	4960..5379	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C3Y93_RS14080	5464..5745	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
C3Y93_RS14085	5746..6048	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3Y93_RS14090	6377..7521	+	1145	WP_104853306.1	IS3 family transposase	-
C3Y93_RS14095	7696..8475	+	780	WP_075594633.1	APH(3')-VI family aminoglycoside O-phosphotransferase	-
C3Y93_RS14100	8627..9652	+	1026	WP_004994718.1	IS30-like element ISAba125 family transposase	-
C3Y93_RS14105	9753..10565	+	813	WP_004201164.1	subclass B1 metallo-beta-lactamase NDM-1	-
C3Y93_RS14110	10569..10934	+	366	WP_004201167.1	bleomycin binding protein Ble-MBL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / aph(3')-VI / blaNDM-1	-	1..38161	38161
	-	inside	IScluster/Tn	mph(E) / msr(E) / aph(3')-VI / blaNDM-1	-	1327..13920	12593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T95305 WP_000286964.1 NZ_CP026617:c6048-5746 [Acinetobacter sp. SWBY1]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T95305 NZ_CP026617:c6048-5746 [Acinetobacter sp. SWBY1]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT95305 WP_000985609.1 NZ_CP026617:c5745-5464 [Acinetobacter sp. SWBY1]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT95305 NZ_CP026617:c5745-5464 [Acinetobacter sp. SWBY1]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94