Detailed information of TA system TA095272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2058821..2059439
• Replicon: chromosome
• Accession: NZ_CP026612
• Organism: Escherichia coli strain DTU-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: C4J63_RS10520
• Protein ID: WP_001290581.1
• Coordinates: 2058821..2059039 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: C4J63_RS10525
• Protein ID: WP_000344800.1
• Coordinates: 2059065..2059439 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4J63_RS10485	2054110..2054682	+	573	WP_000779842.1	YbaY family lipoprotein	-
C4J63_RS10490	2054713..2055024	-	312	WP_000409911.1	MGMT family protein	-
C4J63_RS10500	2055403..2055756	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
C4J63_RS10505	2055798..2057348	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
C4J63_RS10510	2057512..2057982	-	471	WP_000136192.1	YlaC family protein	-
C4J63_RS10515	2058098..2058649	-	552	WP_000102564.1	maltose O-acetyltransferase	-
C4J63_RS10520	2058821..2059039	-	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
C4J63_RS10525	2059065..2059439	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
C4J63_RS10530	2059985..2063134	-	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
C4J63_RS10535	2063157..2064350	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T95272 WP_001290581.1 NZ_CP026612:c2059039-2058821 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T95272 NZ_CP026612:c2059039-2058821 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT95272 WP_000344800.1 NZ_CP026612:c2059439-2059065 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT95272 NZ_CP026612:c2059439-2059065 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5