95258

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1292659..1293576 Replicon chromosome
Accession NZ_CP026610
Organism Bacillus velezensis strain CGMCC 11640

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag C3Z10_RS06710 Protein ID WP_007407256.1
Coordinates 1292830..1293576 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag C3Z10_RS06705 Protein ID WP_003154807.1
Coordinates 1292659..1292829 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3Z10_RS06670 1287882..1289513 + 1632 WP_015417294.1 pyocin knob domain-containing protein -
C3Z10_RS06675 1289526..1289897 + 372 WP_015417295.1 YomQ/XkdW protein, phage-like element PBSX -
C3Z10_RS06680 1289902..1290099 + 198 WP_012117366.1 XkdX family protein -
C3Z10_RS06685 1290156..1290917 + 762 WP_033575307.1 hypothetical protein -
C3Z10_RS06690 1290969..1291232 + 264 WP_003154815.1 hemolysin XhlA family protein -
C3Z10_RS06695 1291246..1291509 + 264 WP_003154813.1 phage holin -
C3Z10_RS06700 1291523..1292401 + 879 WP_007407257.1 N-acetylmuramoyl-L-alanine amidase -
C3Z10_RS06705 1292659..1292829 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
C3Z10_RS06710 1292830..1293576 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
C3Z10_RS06715 1293680..1294678 - 999 WP_003154805.1 inorganic phosphate transporter -
C3Z10_RS06720 1294691..1295308 - 618 WP_003154804.1 DUF47 domain-containing protein -
C3Z10_RS06725 1295594..1296910 - 1317 WP_007610842.1 amino acid permease -
C3Z10_RS06730 1297233..1298183 + 951 WP_012117369.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1258389..1339272 80883


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-239)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T95258 WP_007407256.1 NZ_CP026610:c1293576-1292830 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T95258 NZ_CP026610:c1293576-1292830 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT95258 WP_003154807.1 NZ_CP026610:c1292829-1292659 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT95258 NZ_CP026610:c1292829-1292659 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB I2HQ15


Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References