Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 544113..544750 Replicon chromosome
Accession NZ_CP026610
Organism Bacillus velezensis strain CGMCC 11640

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag C3Z10_RS02660 Protein ID WP_003156187.1
Coordinates 544400..544750 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag C3Z10_RS02655 Protein ID WP_003156188.1
Coordinates 544113..544394 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3Z10_RS02635 540478..541077 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
C3Z10_RS02640 541170..541535 + 366 WP_007410229.1 holo-ACP synthase -
C3Z10_RS02645 541700..542707 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
C3Z10_RS02650 542824..543993 + 1170 WP_039252648.1 alanine racemase -
C3Z10_RS02655 544113..544394 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
C3Z10_RS02660 544400..544750 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
C3Z10_RS02665 544868..545689 + 822 WP_003156182.1 STAS domain-containing protein -
C3Z10_RS02670 545694..546059 + 366 WP_105322051.1 RsbT antagonist protein RsbS -
C3Z10_RS02675 546062..546463 + 402 WP_003156178.1 anti-sigma regulatory factor -
C3Z10_RS02680 546475..547482 + 1008 WP_039252645.1 PP2C family protein-serine/threonine phosphatase -
C3Z10_RS02685 547546..547875 + 330 WP_031378636.1 anti-sigma factor antagonist -
C3Z10_RS02690 547872..548354 + 483 WP_007609591.1 anti-sigma B factor RsbW -
C3Z10_RS02695 548320..549108 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
C3Z10_RS02700 549108..549710 + 603 WP_039252641.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T95257 WP_003156187.1 NZ_CP026610:544400-544750 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T95257 NZ_CP026610:544400-544750 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT95257 WP_003156188.1 NZ_CP026610:544113-544394 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT95257 NZ_CP026610:544113-544394 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References