Detailed information of TA system TA095257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 544113..544750
• Replicon: chromosome
• Accession: NZ_CP026610
• Organism: Bacillus velezensis strain CGMCC 11640

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: C3Z10_RS02660
• Protein ID: WP_003156187.1
• Coordinates: 544400..544750 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: C3Z10_RS02655
• Protein ID: WP_003156188.1
• Coordinates: 544113..544394 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3Z10_RS02635	540478..541077	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
C3Z10_RS02640	541170..541535	+	366	WP_007410229.1	holo-ACP synthase	-
C3Z10_RS02645	541700..542707	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
C3Z10_RS02650	542824..543993	+	1170	WP_039252648.1	alanine racemase	-
C3Z10_RS02655	544113..544394	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
C3Z10_RS02660	544400..544750	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
C3Z10_RS02665	544868..545689	+	822	WP_003156182.1	STAS domain-containing protein	-
C3Z10_RS02670	545694..546059	+	366	WP_105322051.1	RsbT antagonist protein RsbS	-
C3Z10_RS02675	546062..546463	+	402	WP_003156178.1	anti-sigma regulatory factor	-
C3Z10_RS02680	546475..547482	+	1008	WP_039252645.1	PP2C family protein-serine/threonine phosphatase	-
C3Z10_RS02685	547546..547875	+	330	WP_031378636.1	anti-sigma factor antagonist	-
C3Z10_RS02690	547872..548354	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
C3Z10_RS02695	548320..549108	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
C3Z10_RS02700	549108..549710	+	603	WP_039252641.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T95257 WP_003156187.1 NZ_CP026610:544400-544750 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T95257 NZ_CP026610:544400-544750 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT95257 WP_003156188.1 NZ_CP026610:544113-544394 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT95257 NZ_CP026610:544113-544394 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5