Detailed information of TA system TA095201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 170146..170816
• Replicon: plasmid p1
• Accession: NZ_CP026587
• Organism: Klebsiella pneumoniae strain NUHL30457

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: C3483_RS27435
• Protein ID: WP_004213072.1
• Coordinates: 170373..170816 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: C3483_RS27430
• Protein ID: WP_004213073.1
• Coordinates: 170146..170376 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3483_RS27405	166199..167167	+	969	WP_011154535.1	IS5 family transposase	-
C3483_RS27410	167258..167548	-	291	WP_004213078.1	hypothetical protein	-
C3483_RS27415	167900..168106	+	207	WP_004213077.1	hypothetical protein	-
C3483_RS27420	168096..168389	-	294	WP_004213076.1	hypothetical protein	-
C3483_RS27425	168405..169538	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
C3483_RS27430	170146..170376	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C3483_RS27435	170373..170816	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
C3483_RS27440	170830..171246	-	417	Protein_177	restriction endonuclease	-
C3483_RS27445	171250..171543	-	294	Protein_178	transposase	-
C3483_RS30755	171960..172598	+	639	WP_032488580.1	RmpA2	-
C3483_RS27465	173113..173519	-	407	Protein_180	GAF domain-containing protein	-
C3483_RS27475	173610..174530	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
C3483_RS27480	174579..175070	-	492	WP_011251327.1	DM13 domain-containing protein	-
C3483_RS27485	175133..175408	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN / iroD / iroC / iroB	1..215697	215697
	-	flank	IS/Tn	-	-	166199..167167	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T95201 WP_004213072.1 NZ_CP026587:170373-170816 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T95201 NZ_CP026587:170373-170816 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT95201 WP_004213073.1 NZ_CP026587:170146-170376 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT95201 NZ_CP026587:170146-170376 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620