95200

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 42404..43140 Replicon plasmid p1
Accession NZ_CP026587
Organism Klebsiella pneumoniae strain NUHL30457

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag C3483_RS26740 Protein ID WP_004098919.1
Coordinates 42404..42886 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag C3483_RS26745 Protein ID WP_004213599.1
Coordinates 42874..43140 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3483_RS26720 37758..39104 + 1347 WP_011154555.1 dihydroorotase -
C3483_RS26725 39168..40190 + 1023 WP_004214536.1 porphobilinogen synthase -
C3483_RS26730 40365..40760 + 396 Protein_43 DDE-type integrase/transposase/recombinase -
C3483_RS26735 40756..41983 - 1228 Protein_44 IS3 family transposase -
C3483_RS26740 42404..42886 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
C3483_RS26745 42874..43140 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
C3483_RS26750 43335..43565 - 231 WP_004213598.1 hypothetical protein -
C3483_RS26755 43579..43782 - 204 WP_004213596.1 hemolysin expression modulator Hha -
C3483_RS26760 43843..44337 - 495 WP_004213594.1 hypothetical protein -
C3483_RS26765 44379..47348 - 2970 WP_004213592.1 Tn3 family transposase -
C3483_RS26770 47351..47908 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN / iroD / iroC / iroB 1..215697 215697
- inside IScluster/Tn - - 40756..47908 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T95200 WP_004098919.1 NZ_CP026587:c42886-42404 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T95200 NZ_CP026587:c42886-42404 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT95200 WP_004213599.1 NZ_CP026587:c43140-42874 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT95200 NZ_CP026587:c43140-42874 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References