Detailed information of TA system TA095199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 5148..6058
• Replicon: plasmid p1
• Accession: NZ_CP026587
• Organism: Klebsiella pneumoniae strain NUHL30457

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A7D3P3P5
• Locus tag: C3483_RS26540
• Protein ID: WP_023302799.1
• Coordinates: 5588..6058 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: C3483_RS26535
• Protein ID: WP_004026357.1
• Coordinates: 5148..5591 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3483_RS26505	166..687	+	522	WP_004213635.1	sigma-70 family RNA polymerase sigma factor	-
C3483_RS26510	684..1637	+	954	Protein_1	fec operon regulator FecR	-
C3483_RS26515	1724..3850	+	2127	WP_004213640.1	TonB-dependent receptor family protein	-
C3483_RS26520	3925..4080	+	156	WP_004213642.1	hypothetical protein	-
C3483_RS26525	4225..4446	+	222	WP_004213643.1	DUF2188 domain-containing protein	-
C3483_RS26530	4544..5047	+	504	Protein_5	DUF4113 domain-containing protein	-
C3483_RS26535	5148..5591	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
C3483_RS26540	5588..6058	+	471	WP_023302799.1	RES family NAD+ phosphorylase	Toxin
C3483_RS26550	6092..6789	+	698	Protein_8	IS1 family transposase	-
C3483_RS26555	8175..9124	-	950	Protein_9	DUF1173 family protein	-
C3483_RS26560	9302..9796	+	495	WP_004212794.1	thermonuclease family protein	-
C3483_RS26565	10133..10531	+	399	WP_004212796.1	H-NS histone family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN / iroD / iroC / iroB	1..215697	215697
	-	flank	IS/Tn	-	-	6286..6789	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17461.84 Da
• Isoelectric Point: 4.4411

>T95199 WP_023302799.1 NZ_CP026587:5588-6058 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIVWVHHILVMLPTY

Nucleotide

• Length: 471 bp

>T95199 NZ_CP026587:5588-6058 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATTGTATGGGTCCACCACATATTGGTGATGCTGCCAACTTACTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT95199 WP_004026357.1 NZ_CP026587:5148-5591 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT95199 NZ_CP026587:5148-5591 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A7D3P3P5

Antitoxin

• AlphaFold DB: A0A6P1V3Q9