Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 5148..6058 Replicon plasmid p1
Accession NZ_CP026587
Organism Klebsiella pneumoniae strain NUHL30457

Toxin (Protein)


Gene name - Uniprot ID A0A7D3P3P5
Locus tag C3483_RS26540 Protein ID WP_023302799.1
Coordinates 5588..6058 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag C3483_RS26535 Protein ID WP_004026357.1
Coordinates 5148..5591 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3483_RS26505 166..687 + 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -
C3483_RS26510 684..1637 + 954 Protein_1 fec operon regulator FecR -
C3483_RS26515 1724..3850 + 2127 WP_004213640.1 TonB-dependent receptor family protein -
C3483_RS26520 3925..4080 + 156 WP_004213642.1 hypothetical protein -
C3483_RS26525 4225..4446 + 222 WP_004213643.1 DUF2188 domain-containing protein -
C3483_RS26530 4544..5047 + 504 Protein_5 DUF4113 domain-containing protein -
C3483_RS26535 5148..5591 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
C3483_RS26540 5588..6058 + 471 WP_023302799.1 RES family NAD+ phosphorylase Toxin
C3483_RS26550 6092..6789 + 698 Protein_8 IS1 family transposase -
C3483_RS26555 8175..9124 - 950 Protein_9 DUF1173 family protein -
C3483_RS26560 9302..9796 + 495 WP_004212794.1 thermonuclease family protein -
C3483_RS26565 10133..10531 + 399 WP_004212796.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / rmpA / iroN / iroD / iroC / iroB 1..215697 215697
- flank IS/Tn - - 6286..6789 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17461.84 Da        Isoelectric Point: 4.4411

>T95199 WP_023302799.1 NZ_CP026587:5588-6058 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIVWVHHILVMLPTY

Download         Length: 471 bp

>T95199 NZ_CP026587:5588-6058 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATTGTATGGGTCCACCACATATTGGTGATGCTGCCAACTTACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT95199 WP_004026357.1 NZ_CP026587:5148-5591 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT95199 NZ_CP026587:5148-5591 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D3P3P5

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References