Detailed information of TA system TA095180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5383193..5383824
• Replicon: chromosome
• Accession: NZ_CP026585
• Organism: Klebsiella pneumoniae strain WCHKP649

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: BT120_RS28545
• Protein ID: WP_002882817.1
• Coordinates: 5383193..5383576 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BT120_RS28550
• Protein ID: WP_002882814.1
• Coordinates: 5383576..5383824 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BT120_RS28530	5380559..5381461	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
BT120_RS28535	5381458..5382093	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
BT120_RS28540	5382090..5383019	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
BT120_RS28545	5383193..5383576	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
BT120_RS28550	5383576..5383824	-	249	WP_002882814.1	ribbon-helix-helix domain-containing protein	Antitoxin
BT120_RS28555	5384023..5384940	+	918	WP_002882812.1	alpha/beta hydrolase	-
BT120_RS28560	5384954..5385943	-	990	WP_002882811.1	fatty acid biosynthesis protein FabY	-
BT120_RS28565	5385940..5386377	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BT120_RS28570	5386374..5387234	-	861	WP_002882807.1	virulence factor BrkB family protein	-
BT120_RS28575	5387228..5387827	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (18-49)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T95180 WP_002882817.1 NZ_CP026585:c5383576-5383193 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T95180 NZ_CP026585:c5383576-5383193 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9786.10 Da
• Isoelectric Point: 4.3509

>AT95180 WP_002882814.1 NZ_CP026585:c5383824-5383576 [Klebsiella pneumoniae]
MSMMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRK
EW

Nucleotide

• Length: 249 bp

>AT95180 NZ_CP026585:c5383824-5383576 [Klebsiella pneumoniae]
ATGAGCATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCT
CAAAGTGCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTA
AAGATACCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAG
GAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8