Detailed information of TA system TA095165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 741995..742770
• Replicon: chromosome
• Accession: NZ_CP026585
• Organism: Klebsiella pneumoniae strain WCHKP649

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A1Q4Q548
• Locus tag: BT120_RS05125
• Protein ID: WP_004150910.1
• Coordinates: 742285..742770 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: BT120_RS05120
• Protein ID: WP_004150912.1
• Coordinates: 741995..742288 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BT120_RS05100	737126..738037	+	912	WP_004188550.1	LysR family transcriptional regulator	-
BT120_RS05105	738038..739186	-	1149	WP_004150915.1	PLP-dependent aspartate aminotransferase family protein	-
BT120_RS05110	739197..740567	-	1371	WP_004150913.1	cystathionine beta-synthase	-
BT120_RS05115	740952..741649	-	698	Protein_755	IS1 family transposase	-
BT120_RS05120	741995..742288	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
BT120_RS05125	742285..742770	+	486	WP_004150910.1	GNAT family N-acetyltransferase	Toxin
BT120_RS05130	743474..744067	+	594	WP_004188553.1	hypothetical protein	-
BT120_RS05135	744164..744380	+	217	Protein_759	IS3 family transposase	-
BT120_RS05140	744528..744713	+	186	WP_085666571.1	hypothetical protein	-
BT120_RS05145	744655..744876	+	222	WP_004188555.1	hypothetical protein	-
BT120_RS05150	744986..745858	+	873	WP_004188557.1	ParA family protein	-
BT120_RS05155	745858..746241	+	384	WP_004150906.1	hypothetical protein	-
BT120_RS05160	746234..747601	+	1368	WP_004150905.1	replicative DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	740952..744316	3364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17551.59 Da
• Isoelectric Point: 8.8818

>T95165 WP_004150910.1 NZ_CP026585:742285-742770 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Nucleotide

• Length: 486 bp

>T95165 NZ_CP026585:742285-742770 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT95165 WP_004150912.1 NZ_CP026585:741995-742288 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT95165 NZ_CP026585:741995-742288 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A1Q4Q548

Antitoxin

• AlphaFold DB: A0A0H3GVL4